Peptide phosphorodiamidate morpholino oligomers (PPMO) are synthetic DNA mimics that bind complementary RNA and inhibit bacterial gene expression. (RFF)₃RXB- AcpP PPMO (R, arginine; F, phenylalanine; X, 6-aminohexanoic acid; B, β-alanine) is complementary to 11 bases of the essential gene acpP (encodes acyl carrier protein). The MIC of (RFF)₃RXB-AcpP was 2.5...
This publication provides information on the timing and pattern of biomass accumulation and nitrogen (N) uptake for a variety of Pacific Northwest crops. You can use this information to schedule N fertilizer applications for maximum efficiency. To achieve near-maximum crop yields, an adequate supply of available N must be present...
Two different types of Phosphorodiamidate Morpholino Oligomers (PMOs) were used to
inhibit growth in pure cultures of Salmonella enterica serovar typhimurium and in mouse
macrophages infected with S. typhimurium. Both PMOs were 11 bases long and
complementary to bases 6-16 of acyl carrier protein mRNA. A membrane-penetrating
peptide, (RXR)4XB, was...
Cooperative behaviors in bacteria are increasingly appreciated for their relevance to microbial ecology and utility as model systems for social evolution. One example is the secretion of siderophores, a structurally diverse group of compounds that chelate extracellular iron. Siderophore production is considered cooperative because the benefits can be shared with...
Nitrification is the process within the global Nitrogen Cycle where ammonia (NH3) is oxidized to nitrate (NO₃⁻) and can be carried out by two distinct groups of bacteria. The ammonia-oxidizing bacteria (AOB) first oxidize NH₃ to nitrite (NO₂⁻), and second, the nitrite-oxidizing bacteria (NOB) oxidize NO₂⁻ to NO₃⁻. In aerobic...
Nitrification is a critical step in the global nitrogen cycle involving the biological oxidation of ammonia (NH₃) to nitrite (NO₂⁻ ) and then to nitrate (NO₃⁻). The first step in nitrification is carried out by NH₃-oxidizing bacteria (AOB) and archaea (AOA), and the second by NO₂⁻-oxidizing bacteria (NOB). In addition...
Soil acidity, expressed by low soil pH, causes reduced crop growth and significant economic loss. It is the most commonly overlooked and poorly understood yield-limiting factor in western Oregon and a developing concern in eastern Oregon. This publication describes the concept of soil acidity and the limitations it causes for...
Published June 2000. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Amendments and fertilizers applied to optimize crop production and improve soil health also supply inputs of soil organic matter (SOM) and nutrients that affect soil microorganisms. In the dairy-intensive Pacific Northwest, dairy manure is stored as slurry mixtures until conditions are appropriate for soil application. During storage, the manure begins...