This thesis concerns the alteration of the course of selective
protein synthesis and morphogenesis in plants treated with growth
regulating chemicals. Treatment of 48-hour-old "Alaska" pea seedlings
(Pisum sativum L.) with 2, 4-D, IAA, NAA or picloram caused
inhibition of epicotyl and primary root elongation and proliferation of
massed lateral...
Rainbow trout were fed semi-purified diets containing cyclopropenoid
fatty acids, and the biological effects of these acids were
investigated. These fatty acids occur naturally in cottonseed oil and
have been implicated in a large number of physiological disorders.
Included in this study was an investigation of the pattern of deposition...
Malonaldehyde, a very reactive member of the homologous
series of dialdehydes, is associated with the autoxidative deterioration
of lipids. Its measurement, in autoxidized lipid systems is an
expression of the extent of oxidation, in lipids. Malonaldehyde lends
itself well to such determinations because of the sensitivity and
specificity of its...
The biological preparation of aflatoxins was accomplished by
culturing the mold Aspergillus flavus on various media. Aflatoxin
was isolated by chloroform extraction of the culture, and purified
by column fractionation and crystallization. Rice, wheat, soya bean,
peanut and YES media were found to give high yields of aflatoxin. It
was...
The aflatoxins, carcinogenic and toxic mold metabolites
of Aspergillus flavus, were isolated and determined
in cottonseed meal and other feedstuffs. This new
quantitative method uses an acetone soxhlet extraction to
remove the toxins from the defatted meal. The residual
triglycerides, phospholipids and pigments were removed
from the acetone by filtration...
Malonaldehyde, a three carbon dialdehyde, is one of the numerous
carbonyl compounds associated with oxidative deterioration of
food lipids. It is assumed to be formed as a product of lipid autoxidation
occurring according to the generally accepted free radical
mechanism. This compound is of great interest because of its potential...
The variation in fatty acid composition between the "bound" and
"free" lipid in stored anchovy meal was determined using gas-liquid
chromatographic analysis. A model system of fresh anchovy oil on
powdered lactalbumin was also studied to determine the changes in
extractability and fatty acid composition which occur during oxidation.
All...
A method was devised and tested for rapid and quantitative
identification of microbial flora in fresh seafoods. The rapid
identification of large numbers of isolates was made possible by
(1) a simplified identification scheme established by reference
culture studies and from the known reactions of microorganisms
reported in the literature,...
The use of gas chromatography for the separation, comparison,
and subsequent identification of flavor volatiles from food
products has proven highly successful in recent years. The
development of various techniques for the concentration of the
volatiles before gas chromatographic analysis has greatly extended
the use of this important analytical tool....