Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in
which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity.
Thus, we investigated the use of physics-based mathematical optimization to guide design of
cryoprotectant loading methods for mouse and human oocytes. We first...
We combine high-resolution bathymetry acquired using the Autonomous Underwater Vehicle ABE with digital seafloor imagery collected using the remotely operated vehicle ROPOS across the axial valley of the Southern Explorer Ridge (SER) to infer the recent volcanic and tectonic processes. The SER is an intermediate spreading ridge located in the...
Using bulked segregant analysis, five RAPD markers were identified that were
linked in coupling to a gene conferring complete resistance to eastern filbert blight caused
by Anisogramma anomala (Peck) E. Muller in hazelnut (Corylus avellana L.). Two
modified backcross populations were inoculated and scored for resistance to the pathogen
and...
'Marion' blackberry is known for having excellent aroma and flavor, yet they are susceptible to damage by freezing and they are very thorny. Thornless blackberry cultivars have been developed and show great potential. Thus, the interest of the study was in determining which of eight thornless blackberry cultivars, have similar...
Colonization of N. densiflorus tissues by P. ramorum is not well understood. The pathogen is able to colonize nearly all tissues of this host but it is unclear how a tree is ultimately killed. Because this is such a destructive invasive pathogen, it is important to investigate its pathogenic strategy....
The modern world has presented many threats to the health and stability of ecosystems worldwide. One of the most biodiverse ecosystems, coral reefs, faces particularly strong pressures, and is already declining rapidly in complexity and area. Although the stressors that affect reefs are diverse, ranging from nutrient pollution to overfishing,...
Air compressor systems are inefficient energy transfer devices even under the best of conditions, at full load. When only part load is required, efficiency drops further. This thesis attempts to improve part load efficiency of twin rotor screw air compressors in three ways.
First, a guidebook was written to help...
A model of acute glutamate- and domoate-induced toxicity was developed and characterized in cultured rat cerebellar granule cells (CGCs) using experimental conditions which preserve the voltage-dependency of NMDA receptor function. Glutamate, which is normally non-toxic to CGCs in physiologic media (pH 7.4), was shown to induce a cytotoxic response after...