A number of bacterial species are capable of degrading the widespread environmental pollutant trichloroethylene (TCE) via aerobic cometabolism, but cytotoxic effects that can debilitate the microorganism often accompany this transformation. In this dissertation the effects of TCE degradation on the well-studied, toluene-oxidizing bacterium Burkholderia cepacia G4 were investigated at the...
Protein disulfide isomerase (PDI), a member of the thioredoxin superfamily, contains two domains with significant sequence homology to the active sites in thioredoxin. PDI facilitates the folding of nascent proteins in the endoplasmic reticulum (ER), binds hormones and Ca²⁺, catalyzes the glutathione dependent reduction of dehydroascorbate, serves as a major...
Two specific goals were addressed for this dissertation. First to investigate and identify the mechanistic profile of ketoconazole (KT)-induced hepatotoxicity by utilizing in vivo and in vitro approaches determining the mechanism of action for the hepatotoxicity incurred. To date, there has not been a mechanistic determination of the hepatotoxicity associated...
This thesis is an account of investigation on the natural products deriving from various marine algae and has resulted in the discovery of eleven novel bioactive metabolites. Isolation and characterization of these new molecules were carried out using different chromatographic techniques and by analyses of different spectroscopic data, respectively. Using...
Cells of the ventrolateral dermomyotome delaminate and migrate into the limb buds where they give rise to all muscles of the limbs. The migratory cells proliferate and form myoblasts, which withdraw from the cell cycle to become terminally differentiated myocytes. The regulatory mechanisms that control the later steps of this...
This thesis explores the role of Quaker women in science in an attempt to arrive at some understanding of what motivated Quaker women in nineteenth century America to go into the sciences. George Fox founded the Society of Friends in the mid-seventeenth century in England and the Quaker theology centered...
In June 1999, I was a volunteer for a United States non-governmental organization, Crossroads Africa. I joined six other American women traveling to Ghana, West Africa to participate in a collaborative program designed by the Ghana Red Cross Society and Crossroads Africa. Specifically our group was assigned to work on...
Phage-insensitive Streptococcus cremoris starter strains were
selected by assaying cheese whey against potential starter
strains. Six strains were selected and characterized for continual
use in cheesemaking. Upon phage-infection, strains were removed
from the blend. Cheesemaking continued with remaining strains. A
phage-insensitive, fast-acid-producing mutant of the infected
strain was isolated and...
The purposeful induction of malolactic fermentation (MLF) in wines such as Pinot Noir
and Chardonnay is an established commercial wine making practice in Oregon. This induction
is not always successful, especially with white wines, such as Chardonnay. A study was
initiated to examine the compatibility of yeasts commonly used in...