This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:
.
Engineering Bioorthogonal Reactions on Proteins in Live Cells
Melodie M. Machovina and Ryan A. Mehl
Labeling
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:
Cells
Melodie
M.
Machovina
and
Ryan
A.
Mehl
Labeling
of
biomolecules
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:
.
Engineering Bioorthogonal Reactions on Proteins in Live Cells
Melodie M. Machovina and Ryan A. Mehl
Labeling
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:
Cells
Melodie
M.
Machovina
and
Ryan
A.
Mehl
Labeling
of
biomolecules
As a demonstrator for technologies for the next generation of ocean color sensors,
the Hyperspectral Imager for the Coastal Ocean (HICO) provides enhanced spatial and
spectral resolution that is required to understand optically complex aquatic environments. In
this study we apply HICO, along with satellite remote sensing and in situ...
As a demonstrator for technologies for the next generation of ocean color sensors,
the Hyperspectral Imager for the Coastal Ocean (HICO) provides enhanced spatial and
spectral resolution that is required to understand optically complex aquatic environments. In
this study we apply HICO, along with satellite remote sensing and in situ...
Full Text:
Ecology Studies in Monterey Bay, CA, USA
John P. Ryan
1,
*, Curtiss O. Davis
2
, Nicholas B
Widespread habitat degradation and uncharacteristic fire, insect, and disease outbreaks in forests across
the western United States have led to highly publicized calls to increase the pace and scale of forest restoration.
Despite these calls, we frequently lack a comprehensive understanding of forest restoration
needs. In this study we demonstrate...
Full Text:
Landfire BpS Early Seral (A) Mid-Seral Closed (B) Mid-Seral Open (C) Late-Seral Open (D) Late-Seral Closed
Widespread habitat degradation and uncharacteristic fire, insect, and disease outbreaks in forests across
the western United States have led to highly publicized calls to increase the pace and scale of forest restoration.
Despite these calls, we frequently lack a comprehensive understanding of forest restoration
needs. In this study we demonstrate...
Full Text:
/).
⇑ Corresponding author. Tel.: +1 509 248 6672.
E-mail address: rhaugo@tnc.org (R. Haugo).
Ryan Haugo a,⇑, Chris
Diet quality influences ungulate population dynamics but is difficult to measure at fine temporal or spatial resolution using field-intensive methods such as fecal nitrogen (FN). Increasingly, the remotely sensed vegetation index NDVI is used to represent potential ungulate diet quality, but NDVI's relationship with diet quality has yet to be...
Full Text:
Tyler G. Creech a, *, Clinton W. Epps a, Ryan J. Monello b, 1, John D. Wehausen c
a Department of
Diet quality influences ungulate population dynamics but is difficult to measure at fine temporal or spatial resolution using field-intensive methods such as fecal nitrogen (FN). Increasingly, the remotely sensed vegetation index NDVI is used to represent potential ungulate diet quality, but NDVI's relationship with diet quality has yet to be...
Full Text:
Tyler G. Creech a, *, Clinton W. Epps a, Ryan J. Monello b, 1, John D. Wehausen c
a Department of