In vitro growth of twelve isolates of Tither spp., the true truffles, was quantified by an agar-melt procedure. All isolates grew
poorly on media commonly used for the culture of mycorrhizal fungi, but responded markedly to the addition of nitrate, as well as other
inorganic ions, to malt and potato-dextrose...
Replication of plasmid molecules containing either a left-end or a
right-end adenovirus origin was investigated. When the plasmids were
linearized to expose their respective adenovirus termini, mixed, and
transfected with helper adenovirus DNA into the human 293 cell line, a
new molecule was detected. The new molecule was the size...
Polyclonal antisera were raised against isolates of bean
common mosaic virus (BCMV) and bean common mosaic necrosis
virus (BCMNV) using conventional serological methods.
Infected tissues containing, respectively, 22 recognized BCMV
and BCMNV isolates were tested against the two antisera by
antigen-coated plate (ACP) ELISA and double antibody sandwich
(DAS) ELISA....
Experiments were performed to assess regulatory factors governing maize
embryo maturation and vivipary. Both visual and molecular markers of embryo
development were used to examine the roles of the hormones abscisic acid (ABA)
and gibberellins (GAs), as well as water stress in governing transit from early
embryogeny to maturation-phase development....
Maize defective kernel (dek) mutants identify genes necessary for the
successful passage of embryos through the embryogenic and maturation
phases of embryo development. The goal of this thesis was to characterize the
developmental potential of three dek mutants that appeared to be
morphologically blocked prior to the maturation phase of...
Agrobacterium tumefaciens is a gram-negative soil bacterium
that causes crown gall tumors on dicotyledenous plants. The
transferred DNA (T-DNA) portion of the A. tumefaciens tumor-inducing
(Ti) plasmid enters infected plant cells and integrates into
plant nuclear DNA. The T-DNA is accompanied into plant cells by the
VirD2 endonuclease covalently attached...
The work in this thesis has provided conclusive genetic evidence
that "panhandle" intermediates form during adenovirus replication.
Adenovirus chromosomes lacking 51 by from their left -hand termini
are infectious and capable of regenerating the missing origin
sequence. Yet if an entire inverted terminal repeat is removed, the
adenovirus chromosome is...