A comparative study was made on the effect of urea denaturation
of bovine serum albumin (BSA), polyglycyl BSA and poly-L-phenylalanyl
BSA. The denaturation process was followed by ultraviolet
spectrophotometry and optical rotation.
A blue shift in the absorption maximum at 278 mμ was observed
in urea denatured proteins, In 7M...
Independent systems theory has been used to interpret the
circular dichroism (CD) spectra in the vacuum uv of three cyclic
dimers of amino acids. First, the theory for rotational strengths is
developed in terms of degenerate and nondegenerate group functions.
All terms to first order in perturbation theory are kept....
A simple matrix method for the analysis of protein circular dichroism (CD) spectra is presented and used to compute the secondary structure of globular proteins in their native and denatured states. The method uses singular value decomposition and generalized inverse algorithms, and a basis set of CD spectra for proteins...
The association of hyaluronic acid (HA) into a double stranded
structure has been investigated by circular dichroism (CD)
spectroscopy into the vacuum ultraviolet region. The CD of HA changes
dramatically, monitoring a cooperative transition as the dielectric
constant of an aqueous solution is reduced by adding organic solvents.
This structural...
The unfolding of human retinoid X receptor α in the presence of the denaturant guanidine HCl was studied using stopped-flow absorbance spectroscopy. The protein's four native cysteine residues were mutated to obtain four single-cysteine mutants, each with the other three cysteine residues mutated to alanine. The thiols of these hRXRα...
Ellipsometry was used to study the effects of surface energetics
and temperature on the equilibrium adsorptive behavior exhibited by
β-lactoglobulin. β-Lactoglobulin isotherms at 25, 37, and 55°C were
constructed for this purpose. The surfaces of acrylic, polycarbonate,
polyester, glass, and #304 stainless steel were contacted with protein
solutions of varying...