Pseudoperonospora cubensis is an obligate pathogen and
causative agent of cucurbit downy mildew. To help advance
our understanding of the pathogenicity of P. cubensis, we
used RNA-Seq to improve the quality of its reference
genome sequence. We also characterized the RNA-Seq
dataset to inventory transcript isoforms and infer alternative
splicing...
We re-sequenced the Pseudoperonospora cubensis transcriptome to provide the first extensive transcriptome-wide survey of alternative splicing in an obligate biotrophic pathogen during host infection. The libraries from biological replicates of cucumber leaves infected with Ps. cubensis for 2, 3, 4, and 8 days post inoculation (dpi) were re-sequenced using 100-mer...
RNA sequencing (RNA-Seq) is the current method of choice for characterizing transcriptomes and
quantifying gene expression changes. This next generation sequencing-based method provides unprecedented
depth and resolution. The negative binomial (NB) probability distribution has been shown to be a
useful model for frequencies of mapped RNA-Seq reads and consequently provides...
We investigate the value of the correlation function of an inhomogeneous hard-sphere fluid at contact. This quantity plays a critical role in statistical associating fluid theory, which is the basis of a number of recently developed classical density functionals. We define two averaged values for the correlation function at contact...
Snowpack base temperatures vary during accumulation and diurnally. Their measurement
provides insight into physical, biological and chemical processes occurring at the snow/soil interface.
Recent advances in Raman-spectra instruments, which use the scattered light in a standard
telecommunications fiber-optic cable to infer absolute temperature along the entire length of the fiber,...
Many plant-associated Gram-negative bacteria, such a Pseudomonas syringae pv tomato DC3000 (PtoDC3000), use a type III secretion system (T3SS) to establish a host-microbe relationship with compatible plants. The T3SS resembles a molecular injection mechanism that allows the bacteria to deliver type III effectors (T3Es) directly into the cell of the...
Walnuts and hazelnuts: Walnuts and hazelnuts should be dried before eating or storage. Begin the drying process within 24 hours of harvest, especially in the case of walnuts. Nuts usually are dried in the shell, but you can save a considerable amount of drying time and use less heat if...
Stream-dwelling species in the U.S. southern Appalachian Mountains region are particularly vulnerable to climate change and acidification. The objectives of this study were to quantify the spatial extent of contemporary suitable habitat for acid- and thermally sensitive aquatic species and to forecast future habitat loss resulting from expected temperature increases...
Two diametric paradigms have been proposed to model the molecular co-evolution of microbial mutualists and their eukaryotic hosts. In one, mutualist and host exhibit an antagonistic arms race and each partner evolves rapidly to maximize their own fitness from the interaction at potential expense of the other. In the opposing...