Saccharomyces cerevisiae contains three NAD⁺/NADH kinases, one of which is localized in
mitochondria and phosphorylates NADH in preference to NAD⁺. Strand et al (Euk. Cell
2:809 (2003)) reported that a yeast mutation in POS5, which encodes the mitochondrial
NADH kinase, is a mutator, specific for mitochondrial genes. Because of the...
The in planta growth of three wild type strains of phytopathogenic bacteria and three nonpathogenic mutant strains was studied to determine if mutations eliminating the ability of the mutant strains to cause disease had affected their growth in leaf tissue. The mutant strains were obtained by Tn5 mutagenesis of Pseudomonas...
The easy picking characteristic, expressed as low pod detachment force (PDF),
offers a way to decrease pod damage in bush green beans mechanically harvested for
processing and fresh market, and makes hand picking easier and faster. This study was
undertaken to examine inheritance in a cross of two green bean...
An important impact of the genome technology revolution will be the elucidation of mechanisms of cancer pathogenesis, leading to improvements in the diagnosis of cancer and the selection of cancer treatment. Integrated with current well-studied massive knowledge and findings about the role of protein-coding mutations in cancer, demystifying the functional...
Though mutation analysis is the primary means of evaluating the quality of test suites, though it suffers from inadequate standardization. Mutation analysis tools vary based on language, when mutants are generated (phase of compilation), and target audience. Mutation tools rarely implement the complete set of operators proposed in the literature,...
For ras gene mutation analysis in the rainbow trout (Oncorhynchus
mykiss) model system, a partial trout ras sequence was identified
using the polymerase chain reaction (PCR). Two synthetic
oligonucleotides based on rat K-ras gene sequence were used as
primers for the PCR procedure. A 90 base pair (bp) sequence,
referred...
An impediment to use of exotic and bioengineered trees in many places is their propensity for spread by pollen and/or seeds. Our laboratory has been using gene editing to induce mutations in floral genes as means to impart stable and reliable genetic containment when this is desirable from social (markets,...
Snap beans are the vegetable form of dry bean (Phaseolus vulgaris L.) with low fiber, stringless and round, succulent pods that are eaten in the immature stage. Seed companies commit significant resources to maintaining purity and uniformity of snap bean cultivars. While some variability may be introduced through outcrossing (beans...
Integrase catalyzes insertion of a retroviral genome into the host chromosome. Following reverse transcription, integrase binds specifically to the ends of the duplex retroviral DNA, endonucleolytically cleaves two nucleotides from each 3'-end (the processing activity), and inserts these ends into the host DNA (the joining activity) in a concerted manner....
Mismatch repair (MMR) system performs mainly three roles to maintain
genomic stability, correct DNA biosynthetic errors, ensure the fidelity of
genetic recombination, and in mammalian cells participate in the cellular
response to some DNA damages. Deficiencies in mismatch repair increase
mutation rates and cancer risks. In eukaryotes, the MMR system...