Two TaqMan-based real-time One-Step RT-PCR assays were developed for the rapid and efficient detection
of Raspberry bushy dwarf virus (RBDV) and Raspberry leaf mottle virus (RLMV), two of the most common
raspberry viruses in North America and Europe. The primers and probes were designed from conserved
fragments of the polymerase...
Cooling of freshly formed Cheddar
cheese is thought to be one of the processing
steps that requires tighter control
to achieve more uniform and consistent
product quality. Cheese samples, obtained
after pressing, were rapidly cooled
to 5, 15, 25, or 35°C. Commercial samples
and test cheese at 7, 30, 60,...
Parmelina quercina is a well-studied foliose macro-lichen found on rocks and trees in the Northern Hemisphere. Recent studies support multiple species within P. quercina based on material from Europe, North America and western Asia. The identities of Parmelina quercina s.lat. reported from eastern Asia and Alaska remain unknown. We compared...
The placement of ‘Pseudomonas butanovora’ in the genus Thauera was proposed previously, based on 16S rRNA gene sequence analysis, upon further studies of taxonomical characteristics. In this study, physiological characteristics and DNA–DNA reassociation data are presented and the transfer of ‘P. butanovora’ to the genus Thauera is proposed. The original...
The thermal conductivity, measured by the 3ω method, of amorphous films of Al₂P₁.₂O₆ (AlPO) deposited on Si substrates by an all-aqueous spin-coating technique is 0.93(3) Wm⁻¹K⁻¹. The thermal conductivity of a degenerately doped n-Si substrate is 85(5) Wm⁻¹K⁻¹ and of a more lightly doped p-Si substrate is 139(7) Wm⁻¹K⁻¹. The...
In some species, sperm is stored within the female reproductive tract for months to years, and yet remains viable to fertilize eggs and produce offspring. Female red-sided garter snakes store sperm for over seven months of winter dormancy. In previous work we demonstrated that these stored sperm account for an...
Four blackberry (Rubus L. subgenus Rubus Watson) cultivars (‘Obsidian’, ‘Black Diamond’, ‘Metolius’, ‘Onyx’) and two advanced selections (ORUS 1939-4 and ORUS 2635-1) were evaluated during the establishment years of an organic production system for fresh market. The planting was established in Spring 2010 using approved practices for organic production and...
Carbon 1s photoelectron spectra for 2-butyne (CH3C CCH3) measured in the photon energy range from threshold to 150 eV above threshold show oscillations in the intensity ratio C2,3/C1,4. Similar oscillations have been seen in chloroethanes, where the effect has been attributed to EXAFS-type scattering from the substituent chlorine atoms. In...
The uptake of hydrogen by lanthanum pentanickel (LaNi₅) to form lanthanum nickel hydride (LaNi₅H₆) is
followed with three-dimensional imaging by neutron tomography. The hydrogen absorption process is
slower than the time needed for acquiring a single radiograph, about 10 s, but fast relative to the time
to acquire a fully-sampled...