Two methanotrophs, M. trichosporium OB3b and M. buryatense 5GB1, were encapsulated using two methods to investigate the potential of methane conversion for biofuel production. Ca-alginate and low melt agarose were used to immobilize the methanotrophs for batch and continuous flow column testing. Varying protein concentrations, residence times, and immobilization methods...
Reclaimed water is a key source in overall global water management and sustainability;
however, small concentrations of antibiotics in treated wastewater effluent threaten
induction of antibiotic resistance in naturally-occurring soil bacteria and pathogens in
irrigated soils. This type of chronic exposure has the potential to lead to more severe food...
Aromatic hydrocarbons represent a large class of environmental contaminants that have a broad range of structures, physicochemical properties, and toxicities. Arising from the burning of organic matter, particularly fossil fuels, they are both widespread and abundant in all environmental compartments. Both monoaromatic hydrocarbons (BTEX) and polycyclic aromatic hydrocarbons (PAHs) are...
Conventional wastewater treatment facilities using activated sludge, secondary clarifiers, and chlorine disinfection comprise a large portion of urban wastewater treatment practices in the United States. While highly adept at removing chemical, physical, and numerous biological contaminants, these treatment methods are ineffective at removing contaminants of emerging concern, such as antibiotics...
Approximately ninety percent of urban Vietnamese households are connected to septic tanks, from which up to three-quarters of the sludge is reported to be dumped into waterways in residential areas. Vietnam has a considerable prevalence of antibiotic misuse and resultant antibiotic-resistant bacteria (ARB) in the human microbiome. Due to the...
Multi-phase flow in porous media includes many instances of subsurface flow. Three-phase flow in particular is important in situations of enhanced oil recovery, CO2 sequestration, and groundwater remediation. Many studies have been performed on how two fluid phases (oil/water or air/water) behave in porous media, but very few studies exist...
Chlorinated aliphatic hydrocarbons (CAHs) like trichloroethene (TCE) were mostly used to replace drying cleaning fluids which contained hydrocarbons like benzene, and were highly flammable. Through improper disposal, storage, and spills, TCE and its downstream products, like cis-dichloroethene (cDCE) and vinyl chloride (VC), leached into groundwater and threaten human and environmental...
The natural production of methane via anaerobic digestion (AD) is facilitated by a complex community of microorganisms, primarily bacteria and archaea, referred to as the AD microbiome. AD is growing in prominence as a solution to the disposal of food waste, sewage sludge, and the organic fraction of municipal solid...
Acid whey is the largest byproduct of the dairy industry but to date has few useful applications and is frequently discarded as a result. However, acid whey has a high carbon content, making it a suitable co-substrate for anaerobic co-digestion with raw sewage sludge. The synergistic effect of co-digestion has...
Geologic CO₂ sequestration is a climate change mitigation strategy that prevents CO₂ emissions to the atmosphere by capturing CO₂ gasses from large point source emissions streams and then pressurizing and pumping the supercritical-state CO₂ into underground geologic storage reservoirs. Once underground, CO₂ is prevented from buoyant migration to the surface...