2, 4-Decadienal was autoxidized by subjecting a purified sample to an atmosphere of pure oxygen for 14 hours. A qualitative analysis of the autoxidative degradation products was made utilizing tandem gas chromatography and mass spectrometry. Additional information was obtained from the determination of the melting point of the DNP-hydrazone derivative...
A rapid gas liquid chromatographic method was adapted to processed
Dungeness crab for the determination of basic and neutral
volatiles. Methylamine, dimethylamine, and trimethylamine were
measured in processed Dungeness crab for the first time. Generally,
the range for trimethylamine was 42 ppm for frozen crab to 700 ppm
for iced...
Amines formed by biosynthesis in germinated barley have been
suggested as precursors for N-nitrosodimethylamine (NDMA) in barley
malt dried by direct-fired kilning. This hypothesis was verified by
germinating raw barley and exposing the resulting malt roots and
clean malt to nitrous acid. Quantitation of the NDMA formed indicated
that malt...
Porapak Q, Chromosorb 102, and Tenax GC, three porous
polymers commonly used as adsorbents in headspace analyses were
investigated. The retention times of various low-boiling compounds
relative to water were measured on the collection columns to determine
which compounds would be lost during the water removal step
employed after sampling...
The formation of 3-hydroxy-N-nitrosopyrrolidine from decarboxylation
of 4-hydroxy-N-nitrosoproline was demonstrated in model
heating system designed to simulate the conditions of frying bacon.
The identification of the nitrosamine was achieved by comparison of
its TLC R [subscript f] value, gas chromatographic retention time, and mass
spectral fragmentation pattern with those of...
A dose related carcinogenic response was established for dimethylnitrosamine
administered in the diet of rainbow trout (Salmo gairdneri).
An equation was derived for the relationship between dose and hepatocellular
carcinoma incidence. From a published dose response study
using Porton rats as a test animal, a second equation was derived
for...
Dimethylamine (DMA), in addition to the alkaloids hordenine
and gramine, has been suggested as a potential precursor of nitrosodimethylamine
(NDMA) in malt. Analytical procedures were adopted
and applied to extract and quantify DMA from malt, green malt,
malt roots and raw barley. Ground malt was extracted at room
temperature with...
The purpose of this study was to characterize the nonvolatile,
water soluble, bis(hydroxyalkyl)N-nitrosamines produced from the
reaction of spermidine and nitrite.
The biologically occurring polyamine spermidine was reacted
with nitrite in the presence of acid. The reaction products were
isolated and converted to trimethylsilyl (TMS) derivatives. The
derivatized products were...
The malt alkaloids N-methyltyramine, N,N-dimethyltyramine
(hordenine), N-methyl-3-aminomethylindole and N,N-dimethyl-3-aminomethylindole
(gramine) are known precursors for N-nitrosamines.
Analytical procedures were developed and applied to extract and
quantify these alkaloids from malt, green malt, malt roots, and
raw barley. A ground malt sample under nitrogen atmosphere was
extracted with 5 percent concentrated ammonium...