Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in
which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity.
Thus, we investigated the use of physics-based mathematical optimization to guide design of
cryoprotectant loading methods for mouse and human oocytes. We first...
Studies were directed toward the formulation of a selective
Aeromonas medium that would permit the isolation of Aeromonas
species in the presence of other common bacterial flora. The final
composition of this medium, PBG Agar, in grams per liter, is:
Bacto-peptone, 10; Bacto-beef extract, 10; glycogen, 4; NaCl, 5;
sodium...
Marine systems undergo changes in community composition over time as a result of a variety of environmental and anthropogenic factors. Understanding these community changes and the factors that drive them is critical for ecosystem management of marine resources. The Gulf of Alaska (GOA) is a large marine ecosystem that includes...
Six experiments were carried out with Single Comb White Leghorn laying chickens to
assess the effect of feeding a source of direct-fed microbials (Lactobacillus; Lacto) and its
carrier [condensed cane molasses solubles (CCMS)] on the retentions of fat, nitrogen and
several minerals; on the status of the pH of the...
The implementation of advanced hybrid (Monte Carlo/Deterministic) transport methods for realistic test problems has been a challenge due to the overhead efforts associated with interfacing a solution generated by a deterministic solver with a Monte Carlo based radiation transport code. In this work, with the help of Transpire, Inc., a...
The natural production of methane via anaerobic digestion (AD) is facilitated by a complex community of microorganisms, primarily bacteria and archaea, referred to as the AD microbiome. AD is growing in prominence as a solution to the disposal of food waste, sewage sludge, and the organic fraction of municipal solid...
Ethanol production from lignocellulosic feedstock has been under intense scrutiny as a transportation fuel due to its potential to address concerns of increasing energy consumption, limited fossil energy resources, climate changes due to greenhouse gas emissions from fossil fuels, and especially use of non-food biomaterials, which address the biggest limitation...
Radiation exposure to the brain can occur from a variety of events, including space missions, nuclear accidents, and clinical treatments. The effects of high dose radiation are well studied, but those of lower dosage are more elusive. Decline of memory and cognition is observed in patients exposed to low dose...
Food waste (FW), brewery waste (BW), and fats, oils, and greases (FOG), are prevalent waste streams in municipalities across the country, particularly in the Pacific Northwest (PNW). Anaerobic digestion (AD), the microbial-mediated transformation of organic waste into methane-rich biogas, offers a sustainable, energy-generating, waste management solution for these wastes. Anaerobic...
Freshwater systems cycle carbon along a spatial and temporal biogeochemical continuum, across which ecosystem processes contribute to transformations of organic matter (OM). Various ecological constraints impact rates OM transformation and production and consumption of the energetic end of respiration, methane. Microbiological processing and complete reduction of carbon substrates to methane...
Metal oxide clusters are promising inorganic photoresists in next generation nanomanufacturing because of their potential for high-resolution and low line-edge roughness patterning, and exceptional sensitivity to extreme ultraviolet (EUV) radiation. In EUV patterning, absorption of EUV radiation leads to electron emission that serve as a primary species for driving radiation...
State-of-the-art, high-resolution mass spectrometry techniques for acquisition of metabolomic and lipidomic data coupled with advanced computational methods provide new opportunities for interpreting large and complex datasets and comprehending the underlying biological processes of diseases. Both metabolomics and lipidomics strive to obtain comprehensive analyses of small molecules, i.e., metabolites and lipids,...
Freshwater systems are an important component of the global carbon cycle as they outgas disproportionately large quantities of carbon compared to the terrestrial landscape. Of particular importance are headwater streams, which represent roughly 90% of the channel network by length and have been conservatively estimated to outgas roughly 36% of...
Previous studies suggest that vitamin B-6 supplementation can alter fuel metabolism during
exercise and plasma amino acid levels at rest. To examine the effect of vitamin B-6
supplementation on plasma fuel substrates and amino acid levels during exercise, five trained
males (age: 29±7; V0₂ max: 54.7±6.2 ml/kg/min) performed two separate...
The Lean process improvement philosophy has been proposed as a method for improving components of Higher Education (HE), including student learning outcomes; however, current lean applications to HE have focused on administrative operations or general institutional interventions. Comparatively little work has been performed applying lean to teaching. This work fills...
I studied abundance patterns and habitat use of eight cavitynesting bird species in the Coast Ranges of Western Oregon during the
spring and summer of 1985 and 1986. Three age classes of unmanaged Douglas-fir (Pseudotsuga menziesii) stands were selected for study:
young (40-80 yrs), mature (80-200 yrs), and old-growth (>200...
Understanding of gene flow, connectivity, and diversity is critical to predict the stability of key marine species. The Oregon coast of the U.S.A. shows fine-scale levels of geographic variation in environmental stressors such as temperature, pH, and oxygen levels, prompting questions about the potential for local adaptation. In this thesis,...
Water in the U.S. West faces considerable challenges and uncertainties. Climate change has intensified weather extremes, threatening the water sources that support the region’s many stakeholders. Urban water providers are among the stakeholders contending with the challenge of providing reliable water supplies to their growing populations. Recognizing the critical role...
The forest alpine tundra ecotone (FTE, also known as alpine treeline or subalpine parkland), is a conspicuous feature of mountain landscapes throughout the world. Climate change-driven increases in temperature are believed to result in FTE movement and tree invasion of subalpine meadows, which have been documented throughout the Northern Hemisphere...
The hazards-of-place model of vulnerability to environmental hazards posits that vulnerability has biophysical and social components. While biophysical characteristics are important in predicting locations of elevated wildfire risk, the social characteristics of human communities may help us predict locations of elevated wildfire impacts. We examine the relationship between biophysical and...
Heterogeneous catalysis modulates the rate and selectivity of reaction processes by
stabilizing a series of intermediate states on solid surfaces, facilitating the production
of many commodity chemicals modern society relies upon. Conventional, thermal
catalytic processes—conducted at elevated temperatures and pressures—source
thermal, mechanical, and chemical potential energy from carbon-hydrogen bonds—
combustion...
Background: During cellulosic ethanol production, cellulose hydrolysis is achieved by synergistic action of cellulase
enzyme complex consisting of multiple enzymes with different mode of actions. Enzymatic hydrolysis of cellulose is
one of the bottlenecks in the commercialization of the process due to low hydrolysis rates and high cost of
enzymes....
Full Text:
molecular model of enzymatic
hydrolysis of cellulose for ethanol production
Deepak Kumar and Ganti S Murthy
Contributions of sites, rootstocks, and scion clones (and the nteractions between these factors) to scion growth and reproduction were studied in loblolly pine (Pinus taeda L.). Twenty-five full-sib families were used as rootstocks. Six scion clones were grafted on them in all combinations. Study sites were three seed orchards in...
Mathematical modeling plays a pivotal role in understanding the mechanism of radiation-induced cellular effects, and also in quantifying the radiation risk to the cell. However, there are still compelling challenges facing the conventional modeling in radiobiology, such as lacking a generalized theory structure of quantifying target effect and non-target effect...
Anthropogenic semivolatile organic compounds (SOCs) that persist in the environment, bioaccumulate, are toxic at
low concentrations, and undergo long-range atmospheric transport (LRT) were identified and quantified in the atmosphere
of a Saharan dust source region (Mali) and during Saharan dust incursions at downwind sites in the eastern
Caribbean (U.S. Virgin...
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., Majewski, M. S., Foreman, W. T., Genualdi, S. A., Mohammed,
A., & Massey Simonich, S. L. (2014
I tested a theoretical model proposing that anemia
favors transmission of blood-borne parasites to vectors
by accelerating the blood-feeding rate. Using Aedes
aegypti, the yellow fever mosquito, initially, I was not
able to confirm this phenomenon either in an artificial
or a live system; anemia did not correlate with blood-feeding...
Nanoparticles (NPs), defined by their size (1-100 nm), are increasingly incorporated into commercial and industrial products due to their high surface area and unique properties. They can be designed for specific applications by manipulating composition, size, shape, and surface functionalization. As NP production and complexity increases, there is a need...
Molecular genetic and enzymological techniques have been employed to study antibiotic biosynthesis. The nonproteinogenic amino acid capreomycidine is the signature residue found in the tuberactinomycin family of antitubercular peptide antibiotics and an important element of the pharmacophore. Recombinant VioG, a single module peptide synthetase from the viomycin gene cluster cloned...
Zinc oxide nanoparticles (ZnO NPs) have demonstrated the ability to improve lubrication and thermal conductivity, and stand as promising metalworking lubricant and coolant additives due to their low cost compared to other NPs. While nanomaterials are a focus of research due to their potential to enable advanced technologies, little is...
California and Steller sea lions (Zalophus californianus, Eumetopias jubatus, respectively) are considered sentinel species because they have long life spans, are long-term coastal residents, feed at high trophic levels, and have large blubber stores that serve as depots for anthropogenic toxicants. There are few studies on sea lion health and...
This dissertation provides a comparison of statistical and econometric frameworks, using a previously unused freight data source, to study crash frequency by crash type and heavy-vehicle hard braking in Oregon. Hard braking can serve as a proxy for several factors, one of which is safety. Therefore, with the hard braking...
Hydrological and biogeochemical processes occurring throughout a watershed are typically reflected in stream water chemistry, including in coastal watersheds. While Oregon Coast Range streams have been studied in the past, there is a dearth of knowledge in terms of seasonal temporal trends and spatial variability that this study seeks to...
The advent of improved DNA sequencing technologies has allowed the analysis of various microbial communities. Bloom-forming freshwater cyanobacteria can produce toxins and taste-and-odor compounds that can negatively affect drinking water supplies. Here, I have employed second- and third-generation sequencing technologies to characterize bloom-forming freshwater cyanobacterial genomes and their associated heterotrophic...
Echinoderms form an abundant and ecologically important group of marine animals, and they are found in nearly every marine environment, from shallow tropical waters to deep polar benthos and even in the pelagic zone. They exhibit a wide diversity of reproductive strategies that range from broadcasting millions of gametes, with...
Biogas can be used to generate energy from a wide variety of sources. Wastewater treatment sludge is an effective source of waste to be used for generation of biogas to make energy which can be used to heat homes and run businesses. This would help decrease the use of fossil...
Sea otters fulfill a keystone role in several Northeastern Pacific nearshore ecosystems. However, their influence has been absent in Oregon coastal waters since their extirpation in the 19th and early 20th century. Interest has been growing for a translocation attempt to reintroduce sea otters to Oregon from one or more...
This dissertation focuses on indoor free-space optical communications systems for use in short range wireless networks. We propose that current radio frequency wireless links be augmented or replaced with optical frequency links due to overcrowding in the radio frequency spectrum. Optical frequencies contain hundreds of terahertz of unregulated bandwidth and...
Large numbers of hatchery salmon spawn in wild populations each year. Hatchery
fish with multiple generations of hatchery ancestry often have heritably lower
reproductive success than wild fish and may reduce the fitness of an entire population.
Whether this reduced fitness also occurs for hatchery fish created with
local- and...
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Christie, M. R., Ford, M. J. and Blouin, M. S. (2014). On the reproductive success
of early-generation
Primary cultures of stromal-vascular (S-V) cells from adipose tissue were
used to investigate the regulation of preadipocyte development. Differentiation of
S-V cells was found to be under hormonal control. Insulin and glucocorticoids are
essential for S-V cell differentiation in culture.
S-V cells from both newborn and mature pig adipose tissue...
Background: The uneven distribution of recombination across the length of chromosomes results in inaccurate estimates of genetic to physical distances. In wheat (Triticum aestivum L.) chromosome 3B, it has been estimated that 90% of the cross over events occur in distal sub-telomeric regions representing 40% of the chromosome. Radiation hybrid...
Phytophthora infestans, the cause of the devastating late blight disease of potato and tomato, exhibits a clonal reproductive lifestyle in North America. Phenotypes such as fungicide sensitivity and host preference are conserved among individuals within clonal lineages, while substantial phenotypic differences can exist between lineages. Whole P. infestans genomes were...
The purpose of this study was to compare the muscular
strength and endurance of thirty-two men 51 to 79 years of age who
engage in weight training (WT), aerobic training (AT), cross training
(CT), or no training (S). The design employed in the study was a 2x2
between subjects factorial...
Solanum species differ in their degree of frost tolerance and cold acclimation capacity. Cultivated potato species of Solanum tuberosum L. are frost-sensitive, incapable of cold acclimation, and have a maximum freezing tolerance of –3°C. Solanum commersonii Dun is frost-tolerant and can survive to –5ºC pre-acclimation and –10ºC post-acclimation. Breeding attempts...
Bioelution assays are fast, simple alternatives to in vivo testing. In this study, the intra- and inter-laboratory variability in bioaccessibility data generated by bioelution tests were evaluated in synthetic fluids relevant to oral, inhalation, and dermal exposure. Using one defined protocol, five laboratories measured metal release from cobalt oxide, cobalt...
Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
The Lena River in central Siberia is one of the
major pathways translocating terrestrial organic matter (OM)
from its vast catchment area to the coastal zone of the Laptev
Sea and the Arctic Ocean. The permafrost soils of its far
south-stretching catchment, which store huge amounts of
OM, will most...
Based on a literature overview, this paper summarizes the impact and legacy of the contributions of Wilfried Brutsaert and Jean-Yves Parlange (Cornell University) with respect to the current state-of-the-art understanding in hydraulic groundwater theory. Forming the basis of many applications in catchment hydrology, ranging from drought flow analysis to surface...
Falls in older adults are an important concern due to the potential injuries associated with falling. Decreases in muscle strength as a result of natural aging could impair the ability of older adults to prevent either a backward or forward fall. Two musculoskeletal models were used to predict the effect...
The state of Oregon has two distinct climate types bisected by the crest of the Cascade Mountain range. The western side of the Cascades experiences high levels of precipitation and mild temperatures due to the maritime influence of the Pacific Ocean, while the eastern side lies in the rain shadow...
A backward fall is a major cause of hip fractures. Hip fractures have the potential to increase the mortality rate of older adults. Thus, the prevention of backward falls by older adults is a critical subject to be studied. Specifically, it remains unknown what effect aging-related losses in muscle strength...
Wood is a major pool of organic carbon that is highly resistant to decay, owing largely to the presence of lignin. The only organisms capable of substantial lignin decay are white rot fungi in the Agaricomycetes, which also contains non–lignin-degrading brown rot and ectomycorrhizal species. Comparative analyses of 31 fungal...
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, Joseph W. Spatafora, Jagjit S. Yadav,
Andrea Aerts, Isabelle Benoit, Alex Boyd, Alexis Carlson, Alex
Oomycetes form a deep lineage of eukaryotic organisms that includes a large number of plant pathogens which threaten natural and managed ecosystems. We undertook a survey to query the community for their ranking of plant-pathogenic oomycete species based on scientific and economic importance. In total, we received 263 votes from...
It is well known that water transport in xylem depends on its anatomy, but recent research shows that water transport through different parts of the growth ring are affected by the water potential in different ways. The goal of the project was to learn the extent to which wood morphology...
With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
Background: The ability to produce rapid muscle force and joint torque is important for performance in sports, injury prevention and rehabilitation, and balance recovery. One way to measure rapid torque production is rate of torque development (RTD), which is the slope of the torque-time curve. Interventions aimed at increasing RTD...
High Entropy Alloys (HEA) exhibit unusual properties and are gaining interest as new materials for challenging applications in the aerospace, hypersonic and medical fields. The application of HEAs have been limited by the development of powder production at scale. A recently developed process of plasma spheroidization by 6K, inc. has...
The isoprenoid pathway is one of the major biosynthetic pathways of secondary metabolites. Isoprenoids with a C₂₀ skeleton are known as diterpenoids and are derived from the common precursor, geranylgeranyl diphosphate (GGPP). This dissertation describes approaches to discover the diterpene synthase enzymes which provide the backbone structures for pleuromutilin and...
Cell-based biosensors are function-based detectors that use the physiological response of a living cell to sense biologically stimulating agents. This emerging technology extends the application of current detection methods by reporting on the toxicity of a sample and the potential to cause disease. Previously, Betta splendens erythrophores have been described...
Phytophthora sojae is an oomycete pathogen of soybean. As a result of its economic importance, P. sojae has become a model for the study of oomycete genetics, physiology and pathology. The lack of efficient techniques for targeted mutagenesis and gene replacement have long hampered genetic studies of pathogenicity in Phytophthora species....
Recent blackout events consistently show that a variety of mechanisms are involved in cascading outages. These cascading mechanisms are irregularly modeled and validated within the existing literature and industry practices. Understanding the relative significance of these different mechanisms is important for choosing which one(s) needs to be modeled for specific...
Wicking action refers to the movement of a liquid through a porous or a structured surface via capillary action. This phenomenon is affected by surface geometry, such as the wick thickness and pore size of the wick, as well as fluid properties, such as viscosity and surface tension of the...
Current detection methods for bacterial contamination rely on structure based detection of proteins and nucleic acids. While these methods are easy to use and reliable, they cannot evaluate the toxicity of a sample and the potential to cause disease. Previously, erythrophore cells derived from Betta splendens had been suggested as...
Fungi are a large group of eukaryotes found in nearly all ecosystems. More than 250 fungal genomes have already been sequenced, greatly improving our understanding of fungal evolution, physiology, and development. However, for the Pezizomycetes, an early-diverging lineage of filamentous ascomycetes, there is so far only one genome available, namely...
This paper examines the relationship between the university and its regional context with a focus on the case of Nha Trang University and on its potential service role. While the core roles of universities are to impart knowledge and develop human capital, and to promote knowledge creation, the literature also...
Vitrification is a promising approach for cryopreservation of adherent cells because it allows complete avoidance of ice formation. However, high cryoprotectant (CPA) concentrations are required to prevent freezing, and exposure to high CPA concentrations increases the risk of osmotic and toxic damage. Although cell membrane transport modeling can be used...
This thesis is a collection of four submitted manuscripts that present
methods to assist forest ecosystem service managers wanting to develop
operational sampling, monitoring, and production plans for a set of
specific quantifiable ecosystem services, which are formulated as a
series of general multi-objective optimization problems. The problems
are solved...
Alaska-cedar (Chamaecvparis nootkatensIs (D. Don) Spach) is a
valuable tree that is suffering from a serious decline and mortality of
unknown cause throughout southeast Alaska. Epidemiological and
pathological studies were initiated to determine if a pathogen is the
primary cause of this problem. By examining old aerial photographs and
by...
This paper analyses the management of the Atlantic salmon stocks in the Baltic Sea through a coalition game in partition function form. The signs of economic and biological overexploitation of these salmon stocks over the last two decades indicate that cooperation among the harvesting countries, under the European Union's Common...
Due to the many advances in the areas of biotechnology and medicine, the need for long-
term storage and stabilization of biological materials is rapidly increasing. The field of
biopreservation is attempting to address these issues by finding ways to maintain the integrity and
functionality of proteins, cells, and organs...
Mixed anion compounds that form ZrCuSiAs-type crystal structures exhibit remarkable properties such as superconductivity at low temperatures,
hole conduction with optical transparency in the visible region, and exciton absorption at room temperature. In this thesis, properties of a selected group of materials out of the very large set of mixed...
International development programs are often implemented with the goal of building community resilience, but without a clear articulation of how this goal will be met, or any consideration of if or why the effort was successful after it has concluded. This thesis considers three different activities implemented by the U....
This thesis explores the feasibility of using melanophore-based biosensors from Oreochromis niloticus. Melanophores are one type of pigmented cell of the scales and fins of fish that respond in a motile fashion to a diverse range of stimuli. Fish scales were employed as the first step in determining the utility...
Since the introduction of lean manufacturing by Toyota and publication by Womack and Jones, organizations have realized sizeable gains through lean process improvement. The spread of lean practices across organizations and industries – from manufacturing to healthcare and construction – requires adjustments of the lean process and, in the case...
A wealth of information on plant anatomy and morphology is available in the current and historical literature, and molecular biologists are producing massive amounts of transcriptome and genome data that can be used to gain better insights into the development, evolution, ecology, and physiological function of plant anatomical attributes. Integrating...
Successful cryopreservation of all biological specimens would have an untold positive impact on medicine and scientific research. However, cryopreservation of the most complex biological specimens, such as tissues and organs, remains elusive. Vitrification, or ice-free cryopreservation, is promising for cryopreservation of complex specimens. The biggest challenge, though, is the toxicity...
The Atlantic salmon (Salmo salar) is a treasured catch for both commercial and recreational fishers at the Baltic Sea. Especially in Tornionjoki river, which is one of the two remaining wild salmon rivers at the Finnish coast of Baltic, recreational fishing is an increasingly popular activity, and a source of...
The purpose of this honors thesis research project is to investigate the molecular causes of childhood and infant T-cell acute lymphoblastic leukemias. T-cells are the work horses of the immune system, and like any organ, must develop properly to be fully functional. Improper development of thymocytes into T-cells can lead...
Vegetative propagation allows the amplification of selected genotypes for research,
breeding, and commercial planting. However, efficient in vitro regeneration and
genetic transformation remains a major obstacle to research and commercial
application in many plant species. Our aims are to improve knowledge of gene
regulatory circuits important to meristem organization, and...
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., Karlsson, J., Gardestrom, P., Gustafsson, P., Lundeberg, J., and
Jansson, S. (2003). Gene expression in
The g-factor of the 482 Key (Kiloelectron volt) state in Ta¹⁸¹
and the half-lives of the 25.6 Key and 74.5 Key states in Dy¹⁶¹ have
been measured by the delayed coincidence method. The g-factor of
the 482 Key state in Ta¹⁸¹ was determined by observing the effects
of an external...
Successful cryopreservation of bone marrow in situ within vertebral bodies offers an opportunity for quick and convenient storage of a significant quantity of bone marrow stem cells, including hematopoietic- and mesenchymal-type stem cells. However, cryopreservation of more complex large tissues and organs, such as the vertebral body (VB), remains problematic....
Activity‐related knee joint dysfunction is more prevalent in females than males. One explanation
for the discrepancy is differences in movement patterns between the sexes. However the underlying
mechanisms responsible for these differences remain unidentified. This study tested spinal motor
control mechanisms influencing motor neuron pool output and subsequent muscle activation...
Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to...
Four agronomically and genetically diverse spring
barleys were used in a crossing program to study the
inheritance of lysine in barley and to determine its
possible association with certain agronomic and morphological
characters. The cultivar Hiproly was used as the
source of high protein and lysine. Characters measured
included plant...
The objective of this dissertation was to understand the physical mechanisms affecting inversion events in a complex forested mountain landscape. This work was motivated by the long-term studies of climate at the Andrews Forest, short-term studies of vertical temperature, light, wind, and moisture gradient in old-growth trees, and interest in...
In current nanotoxicology research, there is a lack of standardization in reporting of zebrafish exposure media and sonication practices. This lack of standardization in use and reporting methods has made reproducing nanoparticle (NP) toxicity results between labs a common issue. It is important to have a standardized way of reporting...
Post-thinning natural regeneration in the Pacific Northwest of USA was evaluated 13 years after thinning
50-year-old Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco var. menziesii) stands on a drier, interior
Coast Range site (McDonald) and 10 years after thinning 50- to 55-year-old Douglas-fir/western hemlock
(Tsuga heterophylla [Raf.] Sarg.) stands on a moister,...
Discordance in bone mass between young adult swimmers and soccer players may be a direct result of differences in bone loading patterns that influence bone mineralization during growth. Our aim was to evaluate whether sports participation (soccer and swimming) had an independent effect on bone mass accrual at the hip...
A large amount of organic carbon is stored in high-latitude soils. A substantial proportion of this carbon stock is vulnerable and may decompose rapidly due to temperature increases that are already greater than the global average. It is therefore crucial to quantify and understand carbon exchange between the atmosphere and...
The cohesion-tension theory of water transport states that hydrogen bonds hold water molecules together and that they are pulled through the xylem under tension. This tension could cause transport failure in at least two ways: collapse of the conduit walls (implosion), or rupture of the water column through air-seeding. The...
Rainbow trout cystatin C cDNA has been isolated from
trout liver. The full-length cystatin cDNA (674 bp)
included the 5'untranslated region and the polyadenylation
signal sequence AATAAA in the 3' region. Translation of
the cDNA defines 132 amino acid residues. Comparison of
the amino acid sequence with those of family...
This thesis is an integrated study that links several disciplines-archaeology, anthropology, geography, atmospheric sciences, and microbiology. It attempts to generate an argument that central to climate change is disequilibrium in human ecologies- in my case, disease ecologies in Iceland during the 15th century. This thesis investigates the environment's effect on...
Large amount of organic carbon is stored in high latitude soils. A substantial proportion of this carbon stock is vulnerable and may decompose rapidly due to temperature increases that are already greater than the global average. It is therefore crucial to quantify and understand carbon exchange between the atmosphere and...
Characterizing stakeholder behavior when making decisions in watershed management is a great challenge for planners, not only because plans are associated with physical uncertainties but also because stakeholders’ behaviors are influenced by numerous factors. Since stakeholders’ decision-making is linked to multiple types of uncertainty, communication of uncertainty to stakeholders by...
Radioactive In has been incorporated into oriented thin films of YBa₂Cu₃O₇₋δ during a thermal coevaporation process. The hyperfme technique of perturbed γγ-angular correlation spectroscopy shows that 60% of the indium substitutes at a single site in the superconductor. We obtain spectra with reasonable statistics from 2 mCi of starting material,...
Ribonucleotide reductase is an important enzyme in the control of
DNA replication within the cell. Ribonucleotide reductase exerts its
control through enzymatic reduction of nucleoside diphosphates. The
bacteriophage T4 enzyme is an example of the class of iron-requiring
reductases which also includes E. coli and mammalian ribonucleotide
reductases. The two...
A functional test battery (FTB), which combines the use of subjective and objective functional measures, has been proposed as a potential means to distinguish anterior cruciate ligament reconstructed (ACLR) patients' that can more safely participate in activity from those at greater risk. However, no previous study has directly assessed whether...