This thesis involves analyses of 3₂-helices, a-helices and of the change in residual
entropy of a protein upon chemical modification. Part of the long-term goal of
understanding the formation, function and stability of proteins requires that all
conformations be accurately assigned and structurally understood. By a statistical
analysis of 258...
Fluorescence of adenine, guanine, thymine, cytosine, and uracil
has been detected at room temperature in neutral aqueous solution
using a digital signal accumulation technique and right angle fluorescence
detection from solutions with absorbances between 0. 4 and 0. 7.
The quantum yields are respectively 2.6 x 10⁻⁴, 3.0 x 10⁻⁴,...
The binding of eleven polycyclic, aromatic hydrocarbons to the acid
form of poly A has been investigated. The hydrocarbons were chosen to
test a size criterion. Model building showed that some hydrocarbons
could intercalate in the helix and be well protected from contact with
the aqueous solvent. Experimentally it was...
The effects of pH on the torsional flexibility of DNA bound to a
nucleosome core particle were investigated by studying the time-resolved
fluorescence anisotropy decays of ethidium bromide intercalated into the DNA
of the core particle. As the torsional flexibility of DNA is affected by the
presence of an intercalating...
A novel protocol for the study of protein-nucleic acid interactions is presented and demonstrated
to be feasible. The protocol combines photochemical crosslinking techniques and mass
spectrometric methods into a new strategy for identifying protein domains or amino acid residues
that are in close contact with nucleic acid in protein-nucleic acid...
The effects of estrogen treatment on uterine weight, uterine
nucleic acid content and adrenal weight were studied. Daily doses
of 0.05μg or 0.1 μg of Estradiol 17-β were administered subcutaneously
to seven day castrated female rats for periods varying
from 0 to 168 days. After two days of treatment uterine...
The effects of oligoribonucleotides of identical or similar sequences to a
ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly
were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to
the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
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List of Figures
Figure
2.1 The primary and secondary structure of the 16S RNA in E. coli
Nucleic acid estimates in tissues of marine macrophytes with the fluorochromes Hoechst 33258 and ethidium homodimer were severely impaired by high concentrations of interfering compounds. The isolation and extraction techniques used pointed towards compounds of low molecular weight and of polar characteristics that were insensitive to organic solvent extraction and...
Nalidixic acid and its derivatives act through inhibition of
bacterial DNA gyrase activity. Recently there have been a series of
papers reporting the antibacterial activity against three different
types of bacteria (S. aureus, E. coli and P. aeruginosa) by a series
of 1,4-dihydro-4-oxoquinoline (or 1,8-naphthyridine) 3-carboxylic
acids. Consistent biological data...
Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind and silence gene targets. Through designing PPMOs that silence essential or resistance genes in pathogens, these antisense oligomers could be utilized as novel antimicrobials. Towards this end, my thesis employed minimum inhibitory concentration assays (MICs) to identify PPMOs capable of...