Diet History Questionnaires (DHQ) can only provide general information about a person’s nutritional intake. It is unable to cluster groups of people based on diets or lifestyles, nor identify specific foods that may play a role in disease prevention. The aim of this study was to validate the use of...
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polyphenols can lower certain diseases.
Acknowledgements
Special thanks to Fred Stevens, JaewooChoi, Sandra
We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without...
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deficient zebrafish
(danio rerio) embryos
Melissa Q. McDougall a,b, JaewooChoi a, Jan F. Stevens a,c,f
Diet History Questionnaires (DHQ) can only provide general information about a person’s nutritional intake. It is unable to cluster groups of people based on diets or lifestyles, nor identify specific foods that may play a role in disease prevention. The aim of this study was to validate the use of...
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Crystal V. Carrillo1, Rachel Hahn1,2 , Jaewoo Choi2, Gerd Bobe1,2, and Jan F. Stevens Ph.D.2,3
1College of
The protein palmitoylation cycle has been shown to be important for protein signaling and synaptic plasticity. Data from our lab showed a change in the palmitoylation status of certain proteins with age. A greater percentage of the NMDA receptor subunits GluN2A and GluN2B, along with Fyn and PSD95 proteins, were...
We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without...
We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without...
Diet History Questionnaires (DHQ) can only provide general information about a person’s nutritional intake. It is unable to cluster groups of people based on diets or lifestyles, nor identify specific foods that may play a role in disease prevention. The aim of this study was to validate the use of...
To test the hypothesis that embryogenesis depends upon α-tocopherol (E) to protect embryo polyunsaturated fatty acids (PUFAs) from lipid peroxidation, new methodologies were applied to measure α-tocopherol and fatty acids in extracts from saponified zebrafish embryos. A solid phase extraction method was developed to separate the analyte classes, using a...
Skeletal muscle, the largest organ in the body in mass, is composed by 600 specialized muscles in humans with unique biochemical, physiological and metabolic identities. Skeletal muscles control body movement, locomotion, and nutrient balance. Muscle formation requires precisely orchestrated environmental signals and regulatory gene networks in time and space. Gene...
Vitamin E (VitE) is necessary for vertebrate embryonic development. VitE prevents lipid peroxidation (LPO), which requires detoxification by cellular antioxidant systems subsequently involving reducing power derived from energy metabolism. Thus, VitE protects metabolic networks in the developing embryo and the integrated gene expression networks compensating for and impaired by LPO-induced...
Metabolic syndrome, characterized by a collection of risk factors including abdominal obesity, insulin insensitivity, elevated plasma triglycerides, hypertension and low plasma high-density lipoproteins (HDLs), is a major contributing factor to the development of cardiovascular diseases and type II diabetes (T2D). Xanthohumol (XN), a flavonoid isolated from Humulus lupulus cones, has...
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repeated using C57BL/6J female mice
Acknowledgements
Fred Stevens
JaewooChoi
Ralph Reed
Val Miranda
Obesity is achieved at the point where fat accumulation begins to exert deleterious effects on an organism. The human body accrues fat when there is an energy imbalance occurring, where caloric intake exceeds expenditure.Many anti-obesity drugs used in the past have had undesirable side effects, and currently there is a...
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support our hypothesis
24
Acknowledgements
J.F. Stevens, Ph. D.
JaewooChoi, Ph. D.
Val Miranda, Ph. D
Dermatological issues are a widespread problem in humans that are often
overlooked. In our present study, we have isolated lipids from de-identified epidermal
skin tape samples from both Atopic Dermatitis and healthy individuals using a high
efficiency one-step extraction method, which were subsequently analyzed using modified
Liquid chromatography–mass spectrometry (LC-MS)....
Tooth decay in the form of dental caries and cavities is estimated to be among the most common health conditions globally, with treatment of these oral diseases resulting in a massive, world-wide economic burden. The cause of this demineralization of teeth is rooted in the action of complex microbial biofilms...
Delta-sigma analog-to-digital converters traditionally have been used for low speed, high resolution applications such as measurements, sensors, voice and audio systems. Through continued device scaling in CMOS technology and architectural and circuit level design innovations, they have even become popular for wideband, high dynamic range applications such as wired and...
Obesity is achieved at the point where fat accumulation begins to exert deleterious effects on an organism. The human body accrues fat when there is an energy imbalance occurring, where caloric intake exceeds expenditure.Many anti-obesity drugs used in the past have had undesirable side effects, and currently there is a...
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helping me with my cell culture
experiments. I am also deeply indebted to Dr. JaewooChoi for
helping
Atherosclerosis is the underlying cause of ischemic heart disease and stroke and is the leading cause of death worldwide, especially in developed countries. As an inflammatory disease of arteries in a hyperlipidemic milieu, expression of adhesion molecules, such as endothelial-leukocyte adhesion molecule-1 (E-selectin) and intercellular adhesion molecule-1 (ICAM-1), on endothelial...
The use of zebrafish in behavioral neuroscience is rapidly growing. Zebrafish can be assessed for alterations in multiple behavioral endpoints, creating opportunities to use this powerful model to identify chemicals that alter behavioral phenotypes. To evaluate the utility of zebrafish for neurotoxicity research, we designed custom instrumentation to evaluate numerous...
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. ScienceDirect. Web.
Choi, Jaewoo, Scott W Leonard, Katherine
Popular botanical dietary supplements marketed for management of menopausal symptoms, including hops (Humulus lupulus L.), red clover (Trifolium pratense) and licorice (Glycyrrhiza uralensis), were evaluated for their potential to alter CYP-mediated drug metabolism clinically and pre-clinically. Both hops and red clover had been reported to inhibit certain cytochrome P450 (CYP)...
Obesity is a complex multifactorial disease resulting from excessive energy intake compared to energy expenditure. The excess energy is mostly stored as fat in enlarged adipocytes, but some lipids may infiltrate other organs such as the liver to form ectopic fat. Adipose tissue and ectopic fat produce and secrete a...