Biodiversity loss is of global concern, and is due in part to deforestation and high consumer demand for wood and wood products. The neotropical tree species Cedrela odorata (“Spanish cedar” or “cedro”) is economically valuable for its wood and faces threats of overexploitation. Due to strong similarities in wood features...
This thesis summarizes work completed over the previous four years primarily focusing on chloroplast phylogenomic inquiry into the genus Pinus and related Pinaceae outgroups using next-generation sequencing on Illumina platforms. During the time of our work, Illumina sequence read lengths have essentially been limited to 25 to 100 base pairs,...
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Aaron I. Liston RichardC. Cronn
This thesis summarizes work completed over the
Data from nuclear ribosomal internal transcribed spacer regions (nrITS) and chloroplast DNA (cpDNA) have failed to resolve phylogenetic relationships in Pinus. To provide greater interspecific resolution, five low-copy nuclear genes were developed from mapped conifer anchor loci. Four genes were sequenced from species representing all Pinus subsections. Individual loci do...
A fossil-calibrated phylogenetic framework based on exemplars from each of the four taxonomic sections within Pinus was created using multiple nuclear and chloroplast loci. Calibration at the well-defined subgeneric split within Pinus with either fossil leaves and cones (ca. 45 million years ago) or fossil wood (ca. 85 million years...
The study of the infra-specific ranks (i.e., subspecies and variety) can be considered
the study of the process of speciation. Astragalus lentiginosus Douglas ex Hooker
(Fabaceae) is the most taxon rich species in the U.S. flora currently including 40
taxonomic varieties. These varieties were described using traditional taxonomic
methods primarily...
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Rich Cronn Aaron Liston
The study of the infra-specific ranks (i.e., subspecies and
This manual provides guidance on creating subject heading proposals for Indigenous topics that can be submitted to the Subject Authority Cooperative Program (SACO) for addition to the Library of Congress Subject Headings.
Fungi play critical roles in ecosystem processes and interact with plant communities in mutualistic, pathogenic, and commensal ways. Fungal communities are thought to be influenced by both associated tree communities and soil properties. However, the relative importance of the biotic and abiotic drivers of soil fungal community structure and diversity...
The cultivated strawberry, Fragaria Xananassa, is one of the youngest domesticated plants. Its 18th century origin via hybridization in Europe between the North American F. virginiana and the South American F. chiloensis was documented by the botanist Antoine Nicolas Duchesne. His 1766 “Natural History of Strawberries” is an extraordinary work...
Background: Through next-generation sequencing, the amount of sequence data potentially available for phylogenetic analyses has increased exponentially in recent years. Simultaneously, the risk of incorporating ‘noisy’ data with misleading phylogenetic signal has also increased, and may disproportionately influence the topology of weakly supported nodes and lineages featuring rapid radiations and/or...
This dataset is available as supplement for a research article submitted in 2018 and accepted in 2019. Genomic resources for the Neotropical tree genus Cedrela (Meliaceae) and its relatives. By Kristen N. Finch, B.A.; F. Andrew Jones, Ph.D.; Richard Cronn, Ph.D.
Tree species in the genus Cedrela are threatened by...
Rhizopogon is a large genus of ectomycorrhizal (EM) fungi that grows in association with host trees of the family Pinaceae. We have conducted a series of studies investigating the ecology and evolutionary biology of the EM symbiosis shared between R. subgenus Villosuli and trees of the genus Pseudotsuga. Two members...
In recent years, there has been renewed interest in black raspberry (Rubus
occidentalis L.) breeding. This has been spurred, in part, by an increase in black
raspberry consumption due to studies that have shown them to be particularly high in
anthocyanin content indicating high levels of antioxidants. Present cultivars are...
North America, with over 400 species of Astragalus (Fabaceae), is one of three major centers of diversity, all of which comprise the majority of the nearly 1750 species of Astragalus worldwide. One of the most diverse species, Astragalus lentiginosus of Section Diphysi, is a polymorphic complex of over 40 varieties,...
The climate of the Pacific Northwest is in flux, and existing forest ecosystems are stressed and poised to shift in fundamental ways, with or without human intervention. This dissertation probes the nature of forest responses to environmental change through investigations of morphology and genetics of three species of alder co-occurring...
At a time when the biodiversity on Earth is being rapidly lost, new technologies and methods in genomic analysis are fortunately allowing scientists to catalog and explore the diversity that remains more efficiently and precisely. The studies in this dissertation investigate genomic diversity within the milkweed genus, Asclepias, at multiple...
Horizontal gene transfer (HGT) of DNA from the plastid to the nuclear and mitochondrial genomes of higher plants is a common
phenomenon; however, plastid genomes (plastomes) are highly conserved and have generally been regarded as impervious to HGT.
We sequenced the 158 kb plastome and the 690 kb mitochondrial genome...
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consisted of 30 s at 98°C
followed by 25 cycles of 10 s at 98°C, 30 s at a primer set-specific annealing
Horizontal gene transfer (HGT) of DNA from the plastid to the nuclear and mitochondrial genomes of higher plants is a common
phenomenon; however, plastid genomes (plastomes) are highly conserved and have generally been regarded as impervious to HGT.
We sequenced the 158 kb plastome and the 690 kb mitochondrial genome...
Full Text:
(Apocynaceae)
Shannon C.K. Straub1,*, RichardC. Cronn2, Christopher Edwards1, Mark Fishbein3, and Aaron
Horizontal gene transfer (HGT) of DNA from the plastid to the nuclear and mitochondrial genomes of higher plants is a common
phenomenon; however, plastid genomes (plastomes) are highly conserved and have generally been regarded as impervious to HGT.
We sequenced the 158 kb plastome and the 690 kb mitochondrial genome...
Horizontal gene transfer (HGT) of DNA from the plastid to the nuclear and mitochondrial genomes of higher plants is a common
phenomenon; however, plastid genomes (plastomes) are highly conserved and have generally been regarded as impervious to HGT.
We sequenced the 158 kb plastome and the 690 kb mitochondrial genome...
Sequence data from nrITS and cpDNA have failed to fully resolve phylogenetic relationships among Pinus species. Four low‐copy nuclear genes, developed from the screening of 73 mapped conifer anchor loci, were sequenced from 12 species representing all subsections. Individual loci do not uniformly support either the nrITS or cpDNA hypotheses...