Canine Transitional Cell Carcinoma (TCC) makes up 2% of all canine cancers and is the most common cancer of the bladder for dogs. Usually diagnosed in its later stages, TCC is difficult to treat, with low survival times. Current diagnosis involves invasive techniques, and mainstream screening tests are often ineffective....
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Slide 1
Urinary Lipid Biomarkers for
Detecting Canine
Pesticides are among the most pervasive environmental contaminants and they are an important potential risk for human health. Agricultural workers are constantly exposed to pesticide spray, drift and residues in the soil and foliage. Many agricultural pesticides are readily absorbed by the body, through contact with the skin, the respiratory...
Canine Transitional Cell Carcinoma (TCC) makes up 2% of all canine cancers and is the most common cancer of the bladder for dogs. Usually diagnosed in its later stages, TCC is difficult to treat, with low survival times. Current diagnosis involves invasive techniques, and mainstream screening tests are often ineffective....
Full Text:
Urinary Lipid Biomarkers for Detecting Canine Transitional
Oxygenated polycyclic aromatic hydrocarbons (OPAHs) are a class of environmentally prevalent compounds with little known information on their effect in vivo. In a recent study conducted using zebrafish as a model, 38 OPAHs were screened for toxicity. This investigation focuses on two of these OPAHs, 7,12-B[a]AQ and BEZO. Both 7,12-B[a]AQ...
Canine Transitional Cell Carcinoma (TCC) makes up 2% of all canine cancers and is the most common cancer of the bladder for dogs. Usually diagnosed in its later stages, TCC is difficult to treat, with low survival times. Current diagnosis involves invasive techniques, and mainstream screening tests are often ineffective....
Background:
Symmetric dimethylarginine (SDMA) is an accurate and precise biomarker for estimating glomerular filtration rate (GFR) in humans and cats. Serum creatinine (sCr) also correlates with GFR, but has limitations as a biomarker of renal function because nonrenal factors can influence its concentration.
Hypothesis:
Differences in lean body mass (LBM)...
A Sebastes-specific complementary DNA (cDNA) microarray was developed to identify potential biomarkers involved in the capture stress and recovery of Sebastes species if they are assisted in returning to their original depth of capture following barotrauma. Black rockfish Sebastes melanops were exposed to simulated decompression from 450 kPa (c. 35...
Reproductive and endocrine disruption is commonly reported in aquatic species exposed to complex contaminant mixtures. We previously reported that Atlantic killifish (Fundulus heteroclitus) from the chronically contaminated Newark Bay, NJ, exhibit multiple endocrine disrupting effects, including inhibition of vitellogenesis (yolk protein synthesis) in females and false negative vitellogenin biomarker responses...
Transitional cell carcinoma (TCC), the most common cancer of the urinary bladder in dogs, is usually diagnosed at an advanced disease stage with limited response to chemotherapy. Commercial screening tests lack specificity and current diagnostic procedures are invasive. A proof of concept pilot project for analyzing the canine urinary proteome...
Persistent organochlorines (OCs) bioaccumulate in aquatic biota and potentially impair reproduction via endocrine disruption. The present research evaluated estrogenic effects of the OCs, chlordecone (CD) and o,p'-DDT/DDE, and the antiestrogenicity of 3,4,5,3',4',5'-hexachlorobiphenyl (345-HCB) in juvenile rainbow trout, utilizing vitellogenesis as a biomarker. Vitellogenesis, estrogen regulated hepatic production of the yolk...
Osteosarcoma (OSA) is the most common primary skeletal tumor in dogs accounting for 85% of bone tumors and nearly 6% of all canine neoplasms. They are highly aggressive tumors that carry a poor prognosis despite intensive treatment, highlighting the need for more effective diagnostic and therapeutic tools. Recent research in...
Organophosphate insecticides may produce sublethal, adverse effects on salmonid
growth, reproduction, maturation, swimming, and feeding. Chlorpyrifos, an
organophosphate insecticide, was detected by the Oregon Department of Environmental
Quality in the Hood River and tributaries during March 1999 at levels exceeding state
water quality standards in the range of 0.011-0.482 μg/L....
The environmental health science community recognizes polycyclic aromatic hydrocarbons (PAHs) as a re-emerging class of environmental pollutants due to their persistence and prominence in mixtures of concern. Due to their widespread distribution in the environment, exposure to PAHs often occur as complex chemical mixtures. Exposures are linked to numerous adverse...
• Bladder Cancer in the United States has
progressively been one of the more common
cancers and is estimated to have 74,000 new
cases as well as 16,000 deaths in 2015.1
• This type of cancer also has one of the highest
rates of recurrence with an average of 60%...
Heavy biodegraded crude oils have larger numbers of coeluting compounds than non- biodegraded oils, and they are typically not resolved with conventional gas chromatography (GC). This unresolved complex mixture (UCM) has been investigated using comprehensive two- dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-ToFMS) within a set of biodegraded petroleums derived from...
Plant wax lipids and lignin phenols are the two most common classes of molecular markers that are used to trace vascular plant-derived OM in the marine environment. However, their ¹³C and ¹⁴C compositions have not been directly compared, which can be used to constrain the flux and attenuation of terrestrial...
Flavonoids are a large family of compounds synthesized by plants that have a common chemical structure. It is believed that the health effects of fruits and vegetables are primarily due to dietary flavonoids content. Current research indicates that dietary flavonoids show promise for mitigating and/or preventing chronic conditions associated with...
Whey protein’s potential for bioactivity is well documented, but there is disagreement on the extent to which it may modulate the immune system towards decreased inflammation. Decreased inflammation is generally perceived as a beneficial bioactivity in foods as excessive chronic inflammation is linked to morbidity and mortality.
This thesis first...
Atopic Dermatitis (AD) is a widespread skin disorder, affecting some 20% of the world’s population. Though the precise molecular underpinnings are still being understood, changes in lipid composition within the epidermal stratum corneum (SC) layer have been shown to be closely linked with AD. Previous studies have identified several specific...
Determination of the source and fate of natural (higher plant lipids, marine
lipids, etc.) and anthropogenically (e.g., petroleum, coal emissions) derived
hydrocarbons and oxygenated compounds in the environment was accomplished
using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS)
to characterize or identify molecular biomarkers to be utilized as tracers....
Background
As the U.S. population grows older, there is greater need to examine physical independence. Previous studies have assessed risk factors in relation to either disability or mortality, but an outcome that combines both is still needed.
Methods and Results
The Cardiovascular Health Study is a population‐based, prospective study where...
Climate models indicate the Arctic will undergo dramatic environmental change with forecasted increases in temperature and river runoff. Saffron cod (Eleginus gracilis) is abundant in nearshore waters and appears in the diet of many Arctic sea birds and marine mammals; however, little is known about its early ecology and consequently...
Sea surface temperature (SST) estimates were made using data for C37‐39 alkenones analyzed in modern and glacial‐age intervals of sediment cores collected along an east‐west (∼42°N from 125° to 132°W) and a north‐south (41°–33°N) transect of the California Current system (CCS). The prymnesiophyte biomarker results suggest that surface waters warmed...
A sensitive, selective, rapid analytical method based on large-volume injection (LVI)
liquid chromatography/ tandem mass spectrometry was developed using
commercially-available hardware that eliminates the need for either off-line or on-line
solid phase extraction. Centrifugation followed by the direct injection of 1,800 µL
was used for the quantification of illicit drugs,...
Using published dairy cattle liver transcriptomics dataset along with novel blood biomarkers of liver function, metabolism, and inflammation we have attempted an integrative systems biology approach applying the classical functional enrichment analysis using DAVID, a newly-developed Dynamic Impact Approach (DIA), and an upstream gene network analysis using Ingenuity Pathway Analysis...
Estrogen is a sex hormone responsible for the development and regulation of the reproductive system and secondary sex characteristics. They also play a critical role in the development of breast and uterine cancer. The formation of agonistic metabolites of estrogen, especially 16α-hydroxyestrone, have been found to have cancer-inducing effects meanwhile...
Lipid classes, fatty acids (FAs), and stable carbon isotopes of FAs were used to investi-
gate dietary sources of organic carbon for juvenile Atlantic cod
Gadus morhua
during settlement into
eelgrass
Zostera marina
. Primary producers, epibenthic prey, zooplankton, and fish were collected
from August to November 2002 in shallow...
This research was undertaken to develop a sensitive, selective, and rapid analytical method to measure ethyl glucuronide (EtG) and ethyl sulfate (EtS) in urine as urinary biomarkers of alcohol exposure. Large-volume injection (LVI) coupled with liquid chromatography / electrospray tandem mass spectrometry was used to eliminate the need for either...
Female dairy cattle are particularly prone to infertility/subfertility with pregnancy rates ranging as low as 27% to a high 40%. On average it takes more than one breeding/insemination event for dairy cattle to become pregnant. This results in a loss of the number of calves a female would have per...
Oxidative stress occurs when the balance between reactive oxygen species and antioxidant defense mechanisms in the body becomes tipped heavily in favor of the reactive oxygen species. Reactive oxygen species can be present in excess due to elevated O2 levels, radiation, infection, smoking, or even excessive exercise. It is also...
Determining age in crustaceans is inherently imprecise because they molt
periodically and do not retain hard structures throughout their lifespan. Morphological
measurements, such as carapace length, are often used to estimate age because
methods for direct ageing do not exist. However, variability in individual growth rate
and molt frequency can...
During the peripartum period (3 weeks before through 3 weeks after calving, a.k.a. “transition”), high producing dairy cows experience, among others, , oxidative stress and immune suppression that compromise performance and increased incidence of diseases.Among trace minerals, supplementation of Selenium (Se) can help to improve the transition by enhancing glutathione...
Sources of polar/water-soluble organic compounds conjunctly with apolar biomarkers were characterized in natural organic matter. This multi-biomarker approach was accomplished by a simple analytical method consisting of extraction with dichloromethane:methanol (2:1, v/v), silylation and analysis by gaschromatography- mass spectrometry (GC-MS). Polar and apolar biomarkers, derived mainly from higher plants and...
This report examines the feasibility of using the bivalve Corbicula flumina as a biomarker for environmental contaminants in the Willamette River. Compounds such as DDT, dioxins, furans, and PCBs persist in the environment, pose health related concerns, and due to their lipophilic nature accumulate in biological organisms. The success of...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
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biomarkers in sediments are widely used to infer environmental conditions that have 29
occurred in the
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
Lipid biomarkers in sediments are widely used to infer environmental conditions that have
occurred in the geological past, but these reconstructions require a careful consideration of the
biotic and abiotic processes that degrade and alter the lipid biomarker compositions before
and after deposition. In this paper, we use alkenones produced...
