Central Oregon is the primary U.S. hybrid carrot seed production area, supplying seed to the domestic fresh market carrot industry. Approximately 85 percent of the hybrid carrot seed planted in the United States is grown
in Jefferson and adjoining counties. The Madras and Culver areas support most of the carrot...
The ability to faithfully replicate DNA is dependent upon the maintenance
and regulation of its precursors, the deoxyribonucleoside triphosphates.
Enzymes encoded by the bacteriophage T4 have been widely used as models
of biochemical processes. A body of evidence supports the concept that the
bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Many ecologists and environmental scientists witnessing the scale of current environmental change are becoming increasingly alarmed about how humanity is pushing the boundaries of the Earth’s systems beyond sustainable levels. The world urgently needs global society to redirect itself toward a more sustainable future: one that moves intergenerational equity and...
Investigators have been studying an aggregate
containing enzymes of deoxyribonucleoside triphosphate
biosynthesis in T4-phage infected bacteria. They suggest
that it behaves as an organized complex, efficiently
channeling DNA precursors to the replication apparatus.
Previous work has suggested that about 10 enzyme
activities remain associated through several fractionation
steps. This multi-enzyme...
With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
Published October 1978. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Phenylketonuria (PKU) is a genetic inborn metabolic disorder which inhibits the functional production of the enzyme phenylalanine hydroxylase (PAH). It currently affects one in 15,000 Americans and is the most common amino acid metabolism error found in newborns. Individuals affected with PKU are incapable of metabolizing the amino acid, phenylalanine...
Rates of germination and emergence of corn were measured as
influenced by soil physical parameters using blotter paper and a sandy
loam soil as growth media. The corn seeds used were a hybrid of
Funk's (DJ7xLH93).
Experimental conditions were soil temperature of 20°C, 25°C,
30°C, and 35°C, soil water potential...
The nature of inheritance and possible associations for traits
influencing earliness and grain yield were investigated using a four
parent diallel of winter and spring wheat cultivars.
More genetic variability was observed for the traits measured in
segregating populations resulting from crosses between winter and spring
type wheats in contrast...
Published June 2001. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
The purpose of this study was to make a preliminary
investigation of the productivity of some of the soils
within the Willamette catena under different systems of
soil management. The study was carried out at four experimental
sites near Corvallis, Oregon, during the 1962 growing
season. The soil at each...
The influence of variety, plant population, and planting date on
corn silage production was studied at Hyslop Agronomy Farm,
Corvallis, Oregon. The corn plants were grown under irrigation with
300 pounds per acre of 16-20-0 (N-P₂0₅-K₂0) and 240 pounds of urea,
providing a total nitrogen application of 158 pounds per...
Fission product yields (FPYs) are used for a wide range of applications including nuclear fission theory, nuclear reactor design, reactor antineutrino anomaly, stellar nucleosynthesis, and nuclear forensics. These applications rely on FPYs that were last evaluated in 1993, which included measurements from all over the world up until 1993. In...
Wastewater treatment plants are considered one of the main sources and reservoirs of antimicrobial resistance (AMR). This study investigated the prevalence of 8 different phenotypes of AMR, multi-drug resistant (MDR), and extended-spectrum beta-lactamases (ESBL) producing E. coli in 17 wastewater treatment plants across Oregon in winter and summer of 2019...
Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an
enzyme unique to the deoxyribonucleotide metabolism of T-even
bacteriophages. To study biochemical and biophysical properties of
the enzyme, as well as the interaction of dCMP hydroxymethylase with
other DNA precursor biosynthetic enzymes in vitro, availability of large
amounts of the enzyme is...
A crucial factor in determining the accuracy of DNA replication is
maintenance of a balanced supply of deoxyribonucleoside triphosphates
(dNTPs) at replication forks. Perturbation of dNTP biosynthesis can
induce dNTP pool imbalance with deleterious genetic consequences,
including increased mutagenesis, recombination, chromosomal
abnormalities and cell death. Using the T4 bacteriophage system,...
The faithful replication of DNA depends on the appropriate balance of DNA precursors. From studies conducted in bacteriophage T4, models for deoxyribonucleotide biosynthesis producing pools appropriate for DNA replication have made it possible to understand more complex systems. A portion of that body of evidence supports the concept that deoxyribonucleotide...
MIKE SHE is a fully distributed, physically-based hydrologic model that can simulate water movement over and under the Earth's surface. Evapotranspiration (ET) is one of the components of this model. MIKE SHE uses a modification of the Kristensen -Jensen (1975) method to calculate actual ET. This method is based on...
Imazamox-resistant wheat (Clearfield®) cultivars carry the Imi1 gene, which confers resistance to the imidazolinone (IMI) herbicide imazamox. Imazamox provides selective control of jointed goatgrass and other weeds in IMI-resistant wheat. Imi1 gene flow between IMI-resistant wheat and jointed goatgrass may occur via hybridization and backcross events. In 2009 and 2010,...
In this dissertation I explore the Arms representation and activities for teaching quantum mechanics invented at Oregon State University. In the Arms representation students embody quantum states. The Arms activities are presented as an exemplar of the unique physics classroom culture of the upper-division Paradigms in Physics program. In the...
Modern plant pathology benefits from integrating methods and concepts from evolutionary biology. For example, evolutionary concepts are used to identify and examine species boundaries of plant pathogens, recognize processes underlying pathogen biogeography, identify traits that characterize emerging species, and discover new molecular interactions that originate under processes of selection. In...
Bioenergy is a rapidly growing subsector of the emerging global bioeconomy, with the potential to create a substantial number of jobs and mitigate climate change. In order to develop bioenergy into a viable industry, capable of providing valuable energy and employment, there is an immediate need for a skilled workforce...
The trans-Pacific atmospheric transport of particulate matter (PM)-bound polycyclic aromatic hydrocarbons (PAHs) to remote sites in western North America has been well documented and has triggered research questions regarding to atmospheric transformation of PM-bound PAHs and the potential impacts on human health from their inhalation exposure. In this dissertation, field...
The focus of this thesis is to design, characterize, and apply novel computational methods and molecular systems to interrogate heterogeneous human gut microbiome-related phenomena. In Chapter 2, I design, implement, and characterize a method for embedding co-occurrence patterns derived from massive 16s amplicon datasets. I use this method to 1....
Immobilized proteins are integral components of a number of important technologies, including the principal component of many biosensors, as functional coatings in various implants, as catalysts is industrial biosynthesis, and as the building blocks of next-generation nanotechnologies. Despite their ubiquity, relatively little is known about the processes that shape immobilized...
Physics sensemaking is a growing topic of study within the physics education community. Many lenses have been used to study sensemaking but few have considered how time and instruction can impact student sensemaking practices and ideas. In this dissertation, I present four studies that address four major questions: What sensemaking...
Although the Escherichia coli host has almost all of the enzymes necessary to
synthesize nucleotides needed for bacteriophage T4 DNA replication, phage genes
expressed early in infection encode enzymes for de novo DNA precursor biosynthesis
and salvage from degraded host DNA. Eight early enzymes and two host enzymes
comprise the...