There is an urgent need to lower the concentration of sulfur in diesel fuels. Growing concern over environmental effects caused by burning sulfur-containing diesel has led the world to higher standards in fuel refinement. Because of these lower standards, many techniques have been researched to remove sulfur containing compounds or...
Adipic dihydrazide-modified heparin was prepared and
subsequently immobilized on surface-activated silica wafers. X-ray
photoelectron spectroscopy (XPS) was used to quantify the elemental
composition of the silica surface at each stage of activation and results
showed that successful immobilization had taken place. Contact angle
measurements were made to verify the high...
English sole (Parophrys vetulus) is a flatfish found in abundance in the Pacific Ocean and is part of the Pacific Northwest's and California's commercial trawl fishery. Stock levels of this fish are at all time highs but there are decreased landings due to low demand and acceptability. With new regulations...
The three dimensional structure of both a ligand and its cognate receptor are required for the success of structure-assisted drug design. This thesis reports the crystal structure of hectochlorin, a small, bioactive molecule, and the steps toward determining the crystal structure of an RNA molecule that is an attractive target...
A significant body of research demonstrates that phytochemical intake favorably influences hepatic metabolism via a number of different biological mechanisms. Other research has shown a positive effect of certain phytochemicals on kidney health, specifically in ameliorating symptoms of kidney disease. The current research used three separate studies that tested the...
Foodborne outbreaks involving fresh produce have been on the rise since the late 1990's. Pathogens such as Salmonella spp. and Escherichia coli are prevalent in agricultural environments and commonly travel between farms via irrigation water. The Food Safety Modernization Act (FSMA) has placed an increased emphasis on microbiological standards of...
Moderate wine consumption, particularly pigment-rich red wine, is thought by many to be beneficial for the cardiovascular system. Both ethanol and phytochemicals are thought to contribute to this cardioprotective property. Metabolic disturbances associated with overconsumption of food are related to the initiation and progression of many chronic diseases, and cardiovascular...
Metabolic improvements and changes in gene expression were measured in mice fed polyphenol-rich extracts derived from apples and sweet cherries, and the common phytochemicals, quercetin and ellagic acid. Polyphenol-rich extracts were produced by solid phase extraction and column chromatography. Extracts and purified compounds were mixed into a high-fat (HF, 60%...
Sensory evaluation and instrumental methods were applied to the
evaluation of strawberry juice concentrate (68°Brix) stored at
20°C which had been produced both commercially (C-SJC) and in a
pilot plant (SJC). Sensory evaluation included taste and aroma
ratings by intensity scaling and time-intensity of taste, and visual
colorimetry by matching...
The taste qualities of acidulants have not been studied in detail despite
the fact that they are widely used by the food industry. Studies on
characterizing the sensory properties of organic and inorganic acids are very
limited. Reported studies are commonly on threshold, equi-sour and the time
intensity values of...
Effects of moisture content, pH, and salt concentration on dynamic rheological properties and gel fracture quality of Pacific whiting surimi were investigated. Torsion tests showed that shear stress decreased rapidly and strain values decreased gradually as moisture concentration increased. As pH increased, fracture shear stress and strain values increased, whereas...
A focus group interview consisting of 25 international Taiwanese students and a consumer survey in which 793 native Taiwanese urbanites participated were conducted. Both studies investigated the purchasing and consumption behaviors, attitudes, opinions and expectations of Taiwanese people toward cheese. The focus group results provided good predictions and explanations of...
The objectives of this project were to investigate the chemical composition of five varieties of wine grape pomace (WGP) skins obtained in the Pacific Northwest of the United States with the emphasis on dietary fiber (DF) and polyphenolic compounds, and further to evaluate the feasibility of developing WGP extract based...
A number of methods to isolate and quantify protein in Pinot noir wines were evaluated. The combination of precipitation by acetone containing 10% w/v trichloroacetic acid followed by quantification with the Bradford assay, reported in yeast invertase equivalents yielded the most accurate results when compared to micro-Kjeldahl analysis. The technique...
Yeast mannoproteins released during the process of aging wine on the yeast lees have been reported to make important contributions to wine quality. However, few mannoproteins have been identified in wine and their lifespan during aging is unknown. As a first step towards better understanding the contributions of yeast mannoproteins...
High hydrostatic pressure (HHP) affects the pH of weak acids due to an increased degree of acid dissociation while under pressure. The temporary pH reduction by high pressure in such a case may influence the barotolerance of microorganisms. The objective of this study was to determine the impact of weak...
Optimization of preparative nonlinear chromatography was carried out for
the first time for a biomolecule mixture. Conventional wisdom on optimization,
which roots from analytical chromatography, dictates optimizing resolution in an
analytical column and obtaining similar separation in a large column for isolation.
Such a method of optimization significantly under uses...
The studies described in this thesis were motivated by ongoing efforts to develop lignocellulosic biomass as an efficient and practical source of renewable energy. Enormous problems complicate these efforts to reduce reliance on greenhouse gas-generating fossil fuels. Simply obtaining the fermentable sugars available in the cellulose and hemicellulose components of...
Cryopreservation of adherent cells may be advantageous for cell types that are difficult to
preserve in suspension or when it is necessary to preserve characteristics of the adherent cultured cells. Vitrification is a promising procedure for the preservation of adherent cells that prevents ice crystal formation and the resulting dissociation...
The effects of oligoribonucleotides of identical or similar sequences to a
ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly
were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to
the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
Logging and forest thinning operations generate large amounts of residues in the form of small trees, branches and foliage that are usually left on the ground to decay or are burned. These resources are readily available, but it is uneconomical to remove them from the forest. The potential utilization of...
The cholesterol content (mg/100 g wet tissue) of the
longissimus dorsi muscle and the subcutaneous adipose
tissue of "Country Natural Beef" and regularly produced
beef was determined by a spectrophotometric method.
Proximate analysis (moisture, fat and protein contents) of
both types of beef was also determined.
Country Natural Beef (natural...
Effects of whey concentrate crystallization parameters of temperature, pH, and length of time of crystallization on lactose crystal forms and amount of lactose crystallized were investigated. Effect of crystallization parameters on freeze-dried powder quality was also investigated. Lactose crystal forms were observed in x-ray crystal diffraction patterns of freeze-dried whey...
Ceratomyxa shasta is a myxozoan parasite that infects salmonid fishes causing the disease ceratomyxosis that is characterized by severe hemorrhage and necrosis of the intestine and death of the fish host. Ceratomyxa shasta is endemic to the Pacific Northwestern United States and Canada, where epizootics are reported for both wild...
The study of carbonation perception is limited, even though carbonated beverages are very popular. A series of experiments
were conducted to determine the sensory properties of carbonation itself and its interaction with other sensory modalities.
First, the power functions of carbonation in carbonated spring water were developed by a trained...
Analytical chemistry is an essential qualitative and quantitative tool to describe foods and biomaterials and their changes during production, processing and distribution. In this work, rapid analytical procedures for the extraction and quantification of components in fluid milk and diatom fermentation broth were developed. Analytical methods to measure the concentration...