Pretreatment of male C57BL/6 mice with low doses of the persistent organochlorine
(OC) pesticide, chlordecone (CD), stimulated biliary excretion of exogenous CH up to 3-fold. Increased biliary excretion occurred without changes in hepatic ATP-binding
cassette transporter G8 (ABCG8) of the bile canaliculus or scavenger receptor class B
type I (SR-BI)...
Chlordecone belongs to the class of persistent organochlorine pesticides that are remarkably resistant to environmental degradation. Even though their use was banned in the United States in 1978, these compounds can still be detected in both humans and wildlife throughout the world. Previous work has shown that the pretreatment of...
Long-term stimuli of many systems leads to decreased cellular responsiveness, or desensitization. We characterized the desensitization of angiotensin II (Ang 11)-mediated inositol phospholipid (IP) hydrolysis in cultured human aortic smooth muscle cells (HASMC). Although it has been suggested that the desensitization induced by long-term Mg II exposure may result partially...
Salmonid B lymphocytes were stimulated both in vivo and in vitro
and shown to produce antibody specific for the immunogen. A passive
hemolytic plaque assay was employed to demonstrate B cells producing
specific antibody. Characterization of plaque forming cells (PFCs)
involved sequential inhibition with a series of graded concentrations
of...
The in vitro effects of cortisol in regulating salmonid B cell functions were
investigated. B cells at three distinct stages of differentiation were examined for
cortisol sensitivity. B lymphocyte responses were examined 1) during the early stage
of the precursor, 2) during the intermediate stage of differentiation associated with
clonal...
Research in the aviation domain, driving distractions, anesthesia
administration, and nuclear power plant control rooms show that Concurrent Task
Management (CTM) is a process that every human operator performs when
interacting with complex environments. The need for understanding concurrent task
management in a broader perspective more applicable and generalizable to...
The study of physical activity is important in improving people’s health as it can help people understand the relationship between physical activity and health. Accelerometers, due to its small size, low cost, convenience and its ability to provide objective information about the frequency, intensity, and duration of physical activity, has...
The communicative behavior of five species and subspecies of
Tropisternus Solier (Coleoptera: Hydrophilidae) was studied. These
aquatic beetles have stress and calling chirps (both sexes), a second
male calling signal (one species), a single male courtship sound
(usually), a male copulatory sound, and a female aggressive rejection
buzz (three species)....
To characterize the mechanism through which myosin XI-K attaches to its principal endomembrane cargo, a yeast two-hybrid library of Arabidopsis thaliana cDNAs was screened using the myosin cargo binding domain as bait. This screen identified two previously uncharacterized transmembrane proteins (hereinafter myosin binding proteins or MyoB1/2) that share a myosin...
Myosins are actin-based molecular motors that may have specialized trafficking and contractile functions in cytoskeletal compartments that lack microtubules. The postsynaptic excitatory synapse is one such specialization, yet little is known about the spatial organization of myosin motor proteins in the mature brain. We used a proteomics approach to determine...
EF-hand proteins are a conserved family of proteins that are modulated by divalent cations and regulate diverse cellular activities. In the present study we characterized the molecular determinants of myosin regulatory light chain (RLC) interaction with several subunits of the N-methyl-D-aspartate (NMDA)-type glutamate receptor. Myosin RLC is an EF-hand protein...
Urban agriculture (UA) is defined as the production of food crops or livestock within urban areas. Despite its popularity in the United States, research into UA systems suffers from a general underrepresentation of commercial urban systems. As a result, urban growers often have unique technological needs that are unmet by...
The unfolding of human retinoid X receptor α in the presence of the denaturant guanidine HCl was studied using stopped-flow absorbance spectroscopy. The protein's four native cysteine residues were mutated to obtain four single-cysteine mutants, each with the other three cysteine residues mutated to alanine. The thiols of these hRXRα...
It is common to characterize adrenergic receptors by placing
them in one of two categories, alpha or beta. The receptors responsible
for adrenergically induced increase in cardiac contractile force
and glycogenolysis are usually classified as beta receptors, but such
a classification is not definitely established. Therefore, an attempt
was made...
The physical properties of the purified cardiac muscarinic acetylcholine receptor have been examined by D₂O/H₂O sucrose gradient sedimentation and sephacryl S-300 gel filtration in Triton X-405 and dodecyl-β-maltoside. From the sedimentation experiments, the partial specific volume of the receptor-Triton X-405 complex were 0.813 cm³/g and 5.30 S, respectively and the...
Human operator performance was evaluated using two
types of displays in a simulated ocean surveillance task.
The two types of displays studied included a display in
which position uncertainty information was given (polygon
display) and a display in which uncertainty was not
explicitly shown (line display). The experimental task
was...
Research into the effect of automation on human multitasking performance is primarily focused on Decision Automation (a machine suggesting a certain course of action for a human operator to take) and Action Automation (a machine carrying out a certain course of action on behalf of the human operator) while Acquisition...
In today's world, desktop computers have become such an integral part of
our lives that it is practically impossible to imagine anything being done without the
aid of computers. As the world becomes more and more fast paced and users feel a
need to have computers on the go, desktop...
Statistically, travel by air is one of the safest forms of transportation. Yet we
continue to experience aviation tragedies. Do we fully understand flight safety or is
there another way of looking at the problem? In this work a new way of approaching
the question of flight safety is developed,...
Many failures of interpersonal communication and coordination in the aircraft cockpit have been found to occur as a result of poor management of flightdeck 'resources'. Crew Resource Management (CRM) is a concept that has evolved within the aviation community to specifically address this issue of resource management. The concept of...
This research presents a methodology to analyze customer needs and direct the gathering of customer needs that both span the need space and are independent. An ontological categorization, the fundamental research contribution of this work, is created to support both aims of the overall methodology. Providing designers with a customer...
Research in Dolan lab focuses primarily on the adaptive immune system and ways it can be better utilized to eliminate infected or transformed cells. Research focuses on the major histocompatibility (MHC) class I antigen presentation pathway, which plays an important role in alerting the immune system of the presence of...
The present study is an attempt to investigate some of the relationships
between certain personality characteristics and expressed
preferences for various communication structures; it is also an
examination of the relative efficiency of the networks used.
Subjects drawn from the university population participated in
experimentally formed five-person groups. These groups...
When iron levels are low, the opportunistic pathogen Pseudomonas aeruginosa secretes the high-affinity iron-binding siderophore pyoverdine. The cognate ferripyoverdine receptor, FpvA, has gained attention as the most divergent gene in the organism’s genome, showing substantial evidence of positive selection. S-type pyocins, bacteriocins with specificity to Pseudomonads, enter cells through FpvA...
Today’s technology-based society makes information more accessible than ever, in turn creating a growing demand for a workforce that has the skills necessary to utilize data. Contributing to this influx of data is Oregon State University (OSU), who was selected to guide the design and construction process of three Regional...
Gall-associated phytopathogens have unique evolutionary histories that haveshaped both their modes of infection and genomic structures. Pathogenicity of the gall-associated plant pathogens of the Rhodococcus, Agrobacterium, and Rathayibactergenera is mediated by horizontally acquired virulence loci. The relative ease of gainand loss of the virulence loci has confounded accurate characterization of...
Analyses of sympatric and allopatric populations of Sceloporus
occidentalis and S. graciosus from central Oregon indicate a high degree
of similarity in diet. Relative abundance, the equivalent number of
equally common taxa (E), and the probability of similarity (SIMI) were
used in the analyses. Shifts in diet between populations of...
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that is an important regulator of immune responses in various disease models. In the non-obese diabetic (NOD) mouse model of type 1 diabetes, activation of this receptor leads to suppression of the autoimmune response and limits immune cell infiltration of...
Cutaneous melanoma remains the deadliest form of skin cancer, with a
diagnosis of metastasis indicating a median survival rate of less than a year. Solar ultraviolet (UV) radiation, especially childhood sun exposure, is an important etiological risk factor of melanoma. Previous studies determined that mice selectively lacking the nuclear hormone...
The Farnesoid X Receptor (FXR) is a member of the nuclear receptor superfamily of transcription factors that plays a key role in the regulation of bile acids, lipid and glucose metabolisms. The regulative function of FXR is governed by conformational changes of the ligand binding domain (LBD) upon ligand binding....
Research suggests that a key factor in student persistence and success is the relationships students form with faculty (Chickering & Gamson, 1991; Kuh et al., 2005; Tinto, 1987). While it appears to be intuitive that small classes would promote these instructor/student relationships, research on class size provides conflicting results, with...
Currently little is known about the mechanisms and locations of
lymphocyte development in teleosts. In this study several aspects of the
underlying factors which govern B lymphocyte development in trout
were investigated which included: the isolation and characterization of
immunoglobulin heavy chain (IgH) genes, the recombination activating
genes 1 and...
Magnesiothermic reduction can directly convert SiO₂ into Si nanostructures. Despite intense efforts, efficient fabrication of highly nanoporous silicon by Mg still remains a significant challenge due to the exothermic reaction nature. By employing table salt (NaCl) as a heat scavenger for the magnesiothermic reduction, we demonstrate an effective route to...
Most existing methods for generating individual task time estimates in production systems where jobs move through various manually operated workstations remain tedious and time consuming. For existing systems, average task times may vary considerably from prior estimates causing system inefficiencies, poor scheduling and poor planning decisions. Accurate and up-to-date estimates...