Cell signaling under external stimulation is a critical mechanism that governs many biological processes such as cell proliferation, cell migration, and cell apoptosis, etc. For multicellular organisms, the ability to synchronize heterogeneous individual cellular responses through intercellular communication is crucial to maintain normal functionality. However, even though the biological pathways...
At the intersection of basic science research and clinical relevance, cancer metastasis and cell invasion is a multifaceted problem that has been investigated for the past several decades. Importantly, cancer cell plasticity, the ability of a single cell to change its phenotype without genetic mutations in response to environmental cues,...
Instance segmentation, the classification and localization of objects in an image, is a problem in cellular biophysics due to the physiological relevance of cell morphology. Particularly, cancer cells migrate in tissue space by changing their body shape similarly to how humans extend their limbs to swim through water. Monitoring such...
Cell migration is fundamental for physiological processes such as immune re-sponses, wound healing and development. It is also a critical characteristic of metastatic cancer cells. An extensive amount of studies have focused on 2D sub-strates in order to understand the mechanism of cancer metastasis. However, much less is known about...
Cancer cell migration in three-dimensional extracellular matrix is a major cause of death for cancer patients. Although extensive studies have elucidated detailed mechanism of single cell 3D invasion and cell-ECM interaction, 3D collective cancer invasion is still poorly understood. 3D collective migration models have unveiled unexpected degrees of diversity and...
There are clues that suggest human breast cancer cells use collagen fibers to migrate, and vinculin is a transmembrane protein associated with how cells attach to their environment. Therefore, immunostaining for the vinculin will shed some light in how human breast cancer cells are attaching to their environment. Immunostaining for...
The response of cells to periodic driving is important for many biological processes, particularly blood flow and heart functioning. Our research analyzes the collective shear stress response of fibroblast cells due to a periodic driving frequency. When cells experience shear stress, they release calcium into the cytosol. To quantify the...
The structure and mechanics of tissues affect many important cellular functions such as migration, differentiation, and growth. Mechanical interactions between cells and the extracellular matrix (ECM), as well as ECM-mediated mechanical communication between cells, plays a part in coordinating collective cellular dy- namics during critical processes such as morphogenesis, tissue...
The ability for individual cells and multicellular networks to process information contained in perceived stimuli is of vital importance to their proper functioning and ultimately their cellular fate. The primary mechanism employed by cells to this end is the use of biochemical signaling in which a series of molecular events...
Cancer is a hard disease to control because of its ability to spread to different parts of the body. Cancer cells can spread out from a primary tumor to distant organs which gives rise to new cancer cell colonies and more tumors over time. This process is known as metastasis...
Current methods in traction force analysis rely on a homogeneous extra-cellular matrix and
embedded microbeads to track deformations. The efficacy of these methods decreases when the matrix is not homogeneous. In heterogeneous matrices, beads often aggregate in regions of higher matrix density and float in regions where matrix substrate is...
Invasive breast cancer affects 1 in 8 women during their lifetime. Current cancer research uses 2-D cell culturing, which does not accurately represent tumor behavior. In comparison to tumors, cells grown using 2-D cell culturing have different proliferation and diffusion rates, cell shapes, and gene expression. To improve in vitro...
We developed an integration-based line detection algorithm. Existing line detection methods such as the Hough Transformation (HT) and its variants are insensitive to image noise. The reason is that HT finds lines by calculating the gradient of the image and assumes that the region where the gradient is the steepest...
We developed an integration-based line detection algorithm. Existing line detection methods such as the Hough Transformation (HT) and its variants are insensitive to image noise. The reason is that HT finds lines by calculating the gradient of the image and assumes that the region where the gradient is the steepest...
- Collagen fibers are found in 25% of the whole body
protein content. They are highly adhesive to cells and
can be used as scaffold proteins that can promote cell
growth and be used to capture cells.
- In our lab, we use collagen to capture cancer cells in
order...
This research specifically examines the collective motion of populations of MDA-MB-231 breast cancer cells under the influence of a chemo-attractant gradient. Their motion is compared to the mathematical model of a persistent random walk, measuring the persistence bias of the cells from their autocorrelation function and applying to the mean...
Many biological processes are regulated by the presence and movement of cellular Ca²⁺ ions. The concentration of Ca²⁺ in a cellular environment is regulated by IP₃ sensitive channels that lie on the surface of a cell’s endoplasmic reticulum. Little is known about the macroscopic effects of intracellular Ca²⁺ activity, so...
Exploring and quantifying the parameters regarding contact guidance may provide valuable insight to the precise mechanisms controlling cell movement, especially in a three-dimensional environment. The objective of this project was to quantify the relationship between MDA-MB-231 cell morphogenesis and the geometry of the cell’s local microenvironment, which was modeled by...
Forward osmosis provides a low energy alternative for waste water purification. One of the major issues facing this process is the formation of a foulant layer preventing fluid transfer across the membrane. In recent studies, a conductive layer added to the membrane surface allowing an electric charge to be present...
Type I collagen fibers, the connective tissue that makes up the human breast, can be easily manipulated by cells or other outside factors. In previous studies, the alignment of collagen has been associated with the beginning of metastatic cancer, possibly influencing cancerous cells to migrate from the tumor. In this...