Tn5 mutagenesis of apdA (for antibiotic production) and deletion of
gacA (for global antibiotic and cyanide) resulted in the same pleiotropic phenotype in
Pseudomonas fluorescens (i.e. production of an array of secondary metabolites
including the antibiotics pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol as
well as a tryptophan-side-chain oxidase, hydrogen cyanide, and an...
The objective of this dissertation is to enhance the monitoring of forest ecosystems through the utilization of remotely sensed data to address the exigencies posed by the Anthropocene. On a global scale, rising temperatures and fluctuating precipitation patterns have strained forests and produced shifts in natural disturbance regimes. Additionally, the...
Emerging infectious diseases are increasing globally and are a threat to human, wildlife, and ecosystem health. The emerging fungal pathogen, Batrachochytrium dendrobatidis (Bd), or amphibian chytrid fungus, is associated with worldwide amphibian population declines and extinctions. Bd has been found on every continent where amphibians exist and has been documented...
Chlamydiae are obligate intracellular bacteria that infect a variety of
eukaryotic hosts and affect normal host processes. Within host cells, their
developmental cycle takes place inside non-acidified vacuoles termed inclusions. An
inclusion membrane composed primarily of secreted chlamydial synthesized proteins
called Incs encloses the inclusion. At this location, Incs have...
The Safe Drinking Water Act ensures that public systems provide water that meets health standards. However, no such protection exists for millions of Americans who obtain water from private wells. Concern for safety is warranted as most wells draw from underground aquifers, and studies demonstrate that groundwater is affected by...
In this thesis I present the results of a comprehensive assessment of the Pacific-North American (PNA) teleconnection pattern in general circulation models (GCMs) and a regional climate model (RCM). The PNA teleconnection pattern is a quasi-stationary wave field over the North Pacific and North America that has long been recognized...
Regionalized variable analysis has been used to study
a number of meteorological and hydrological variables
including precipitation (Delhomme and Delfinier, 1973),
streamfiow (Villeneuve et al., 1979) and hydraulics of
groundwater aquifers (Gambolati and Volpi, 1979). In the
research work reported in this thesis, an attempt was
made to characterize the...
This book is based on a project to completely annotate what is known about the function of the ~150 genes encoded by the genome of the most commonly investigated baculovirus, AcMNPV. Once the annotation was completed (summarized in Chapter 12), the material was organized into a series of chapters integrating...
This is the third edition of a book that was initiated with the annotation of the function of all the genes in the most commonly studied baculovirus, AcMNPV. The second and third editions involved the update of this information and its integration into chapters covering the major processes central to...
This is the 4th edition of a book that was initiated with the annotation of the function of all the genes in the most commonly studied baculovirus, AcMNPV. It has been almost six years since I reviewed this literature. As a measure of the research that has occurred over this...
Proceedings of Visible Possibilities: The Economics of Sustainable Fisheries, Aquaculture and Seafood Trade, the 16th Biennial Conference of the International Institute of Fisheries Economics & Trade, held July 16-20, 2012 in Dar es Salaam, Tanzania.
Ultrafast spectroscopy can exploit the structure-function relationship of chemical- and biological-based systems. More specifically, femtosecond stimulated Raman spectroscopy (FSRS) provides ground- and excited-state vibrational information with simultaneously high spectral (spatial) and temporal resolutions. The electronic counterpart, femtosecond transient absorption (fs-TA), complements FSRS data analysis as part of a comprehensive approach...
The sexually transmitted disease gonorrhea, caused by the Gram-negative bacterium and obligate human pathogen Neisseria gonorrhoeae, remains a significant health and economic burden worldwide. In the absence of a protective vaccine, antimicrobial agents are the only pharmacological intervention for patients with gonorrhea. However, due to the remarkable ability of gonococcus...
Based on current trends both pesticide and plastic production are expected to continue to rise in the coming decade. A growing global population coupled with the effects of climate change are the main drivers of increased pesticide use. Throughout this dissertation pesticides refer to herbicides, fungicides, and insecticides. Plastic production...
On a global scale, potato (Solanum tuberosum L.) plays an important role in tackling the threat of food insecurity due to its high yield and broad global acceptance. However, pathogens threaten potato production, causing direct yield loss and rendering potatoes tubers unmarketable. Breeding new cultivars that carry multiple resistances is...
The advent of improved DNA sequencing technologies has allowed the analysis of various microbial communities. Bloom-forming freshwater cyanobacteria can produce toxins and taste-and-odor compounds that can negatively affect drinking water supplies. Here, I have employed second- and third-generation sequencing technologies to characterize bloom-forming freshwater cyanobacterial genomes and their associated heterotrophic...
The concept of compensatory mitigation is well established as an approach to environmental management. In the past, mitigation programs have been used to conserve wetlands affected by development, and is proposed as a cost-effective approach to offsetting greenhouse gas emissions through reforestation programs. The concept may be equally applicable to...
Emerging infectious diseases impact both human and wildlife populations. Infectious agents, in particular the aquatic fungus Batrachochytrium dendrobatidis (chytrid), have an influential role in driving global amphibian population declines. The emergence of the chytrid fungus has aspects of both geographic spread as well as climate shifts altering environmental conditions and...
Dissolved organic matter (DOM) in the oceans is one of the largest pools of reduced carbon on Earth, comparable in size to the atmospheric CO₂ reservoir. A vast number of compounds are present in DOM, and they play important roles in all major element cycles, contribute to the storage of...
Chloroplast genomes of conifers are unusual in a
number of respects, including the possession of much
repetitive DNA. By studying the distribution and sequences
of dispersed repetitive DNA, I hoped to gain insight into
the mechanisms of chloroplast genome evolution.
I used restriction mapping and DNA sequencing to
characterize dispersed...
European hazelnut, Corylus avellana L., is the only economically important nut crop
in the family Betulaceae. One of the threats to the hazelnut industry in the Pacific
Northwest is the fungal disease eastern filbert blight (EFB) caused by the
pyrenomycete Anisogramma anomala. Host genetic resistance to EFB identified in the...
The deep sea is often viewed as a vast, dark, remote,
and inhospitable environment, yet the deep ocean and
seafloor are crucial to our lives through the services that
they provide. Our understanding of how the deep sea functions
remains limited, but when treated synoptically, a diversity
of supporting, provisioning,...
Electrophoretic karyotypes were obtained from intact cells of four wild
type strains of Tilletia caries and T. controversa, and 5 inter specific hybrid
progeny using clamped homogeneous electric field, pulsed field gel
electrophoresis (CHEF PFGE). Each karyotype was polymorphic and unique
relative to the other strains. However, the relative size...
All animals have developed the critical ability to detect, respond to, and detoxify a large array of environmental chemicals and stressors that can cause adverse health effects. Important examples of landmark contaminants around the world are polycyclic aromatic hydrocarbons (PAHs) and dioxins, both of which can act via the aryl...
The objective of this research is to support DfX considerations in the early phases of design, particularly Design for the Environment. In order to include DfX considerations, especially early in the design process, there needs to be access to knowledge and information that is usually only applicable further downstream in...
Analytical chemistry is an essential qualitative and quantitative tool to describe foods and biomaterials and their changes during production, processing and distribution. In this work, rapid analytical procedures for the extraction and quantification of components in fluid milk and diatom fermentation broth were developed. Analytical methods to measure the concentration...
Final program details of Visible Possibilities: The Economics of Sustainable Fisheries, Aquaculture and Seafood Trade, the 16th Biennial Conference of the International Institute of Fisheries Economics & Trade, held July 16-20, 2012 in the Hyatt Regency Kilimanjaro Hotel, Dar es Salaam Tanzania
Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. In the first study, 12 robust Simple Sequence Repeat (SSR) markers were developed from two microsatellite-enriched libraries of...
In this report, we present climate projections for Morrow County that are relevant to specified natural hazards for the 2020s (2010–2039) and 2050s (2040–2069) relative to the 1971–2000 historical baseline. The projections are based on multiple global climate models for both a lower greenhouse gas emissions scenario (RCP 4.5) and...
In this report, we present future climate projections for Polk County relevant to specified natural hazards for the 2020s (2010–2039) and 2050s (2040–2069) relative to the 1971–2000 historical baseline. We present projections that are based on multiple global climate models for both a lower greenhouse gas emissions scenario (RCP 4.5)...
In this report, we present climate projections for Union County that are relevant to specified natural hazards for the 2020s (2010–2039) and 2050s (2040–2069) relative to the 1971–2000 historical baseline. The projections are based on multiple global climate models for both a lower greenhouse gas emissions scenario (RCP 4.5) and...
This work explores a framework for the gathering and analysis of information rich customer needs, with the goal of informing inclusive product design practice. The goal is to be able to provide information and experience to designers so that they can better understand the needs of exceptional users and include...
Staphylococcus aureus is a human pathogen capable of producing enterotoxins causing staphylococcal food poisoning, one of the most economically important foodborne diseases in the U.S. This pathogen may be introduced to pre-cooked tuna meat used for the production of canned tuna because significant manual handling occurs after cooking of the...
European hazelnut (Corylus avellana L.) is a diploid with 11 pairs of chromosomes (2n = 2x = 22), monoecious, dichogamous and wind-pollinated. Eastern filbert blight (EFB) caused by Anisogramma anomala is a serious disease of European hazelnut (Corylus avellana) in North America, and infection leads to cankers that girdle branches...
OmniGen-AF® (OG; Prince Agri Products, Inc., Quincy, IL) is a feed additive containing brewers dehydrated yeast and B-vitamins that supports immune function in ruminant livestock. Targeted profiling of immune-associated genes in whole blood is an effective platform for identification of a) novel molecular pathways and b) immune response markers to...
Fungi and Actinobacteria are essential actors in global biogeochemical cycles of carbon and nutrients, and have both been recently appreciated for their roles in marine ecosystems. However, the diversity and distribution of these ubiquitous, but low-abundance microbes in deep-sea habitats remains poorly understood, particularly in chemosynthetic habitats such as methane...
Direct seeding techniques often result in unsatisfactory outcomes in rangeland rehabilitation, primarily because of low seedling emergence and poor establishment. Seed processing techniques aimed at improving seedling emergence have gained interest by pasture managers. The purpose of this study was to investigate the combined effects of bracteole removal and seeding...
Capacity reduction programs in the form of buybacks or decommissioning programs have had relatively widespread application in fisheries in the US, Europe and Australia. A common criticism of such programs is that they remove the least efficient vessels first, resulting in an increase in average efficiency of the remaining fleet....
Two studies were conducted to evaluate the influence of supplement composition on intake and digestibility of a low-quality (< 6% CP), cool-season forage, as well as cow performance. Treatments included a non-supplemented control (CON), corn (approximately 8% CP), corn and urea (LU = corn + 0.09 mg/kg BW urea, approximately...
Small-scale fisheries (SSF) around the world face many challenges. They are a highly dynamic, important sector for coastal communities in developing nations, playing a critical role in poverty alleviation and food security. SSFs generally have few resources to ensure their long-term sustainability. They are often fished and managed locally and...
Specialized or secondary metabolism is a collection of pathways and small molecules that, while beneficial to an organism, are not strictly necessary for survival. Plants use secondary metabolites to, among other things, attract pollinators, defend against biotic and abiotic stressors, and form symbioses. Natural products from plants have seen an...
Vibrio parahaemolyticus is a Gram-negative, halophilic, human pathogenic bacterium ubiquitous in the marine environment. Like many Vibrio species, V. parahaemolyticus commonly associates with shellfish, particularly oysters. Ingestion of a raw or under cooked oysters contaminated with V. parahaemolyticus can cause gastroenteritis, which is typically self-limiting and rarely causes death. Globally,...
The objectives of this project were to investigate the critical factors impacting the physicochemical and antibacterial properties of β-chitosan based films derived from jumbo squid (Dosidicus gigas) pens, and to evaluate the feasibility of improving water solubility of β-chitosan through Maillard reaction. The studies examined the effect of molecular weight...
T cell-dependent immune responses are highly sensitive to suppression by
exposure to the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin
(TCDD), yet direct effects of TCDD on T cells have been difficult to demonstrate.
Because the activation of naive T cells is initiated by dendritic cells (DC), the studies
presented in this dissertation were...
The immune toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been studied for over 35 years, but only recently has the profound immune suppression associated with TCDD exposure been linked to induction of regulatory T cells (Tregs). The effects of TCDD are mediated through binding the aryl hydrocarbon receptor (AhR), a ligand-activated transcription...
Australia has a policy of achieving maximum economic yield (MEY) in Commonwealth fisheries, with many States also interested in the MEY target. Bioeconomic models are being developed for estimating MEY for several fisheries, supported by economic surveys of the fisheries. While most cost components can be derived directly from the...
Metal oxide clusters are promising inorganic photoresists in next generation nanomanufacturing because of their potential for high-resolution and low line-edge roughness patterning, and exceptional sensitivity to extreme ultraviolet (EUV) radiation. In EUV patterning, absorption of EUV radiation leads to electron emission that serve as a primary species for driving radiation...