The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a
long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation
of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Full Text:
Fluorescent Amino Acid for Lifetime and
FRET/LRET Studies
Lee C. Speight,† Anand K. Muthusamy,† Jacob M
The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a
long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation
of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Full Text:
-ylalanine, a
Fluorescent Amino Acid for Lifetime and FRET/LRET Studies
Speight, L. C., Muthusamy, A. K