Rhizopogon salebrosus is an abundant ectomycorrhizal fungus common throughout Western North America that forms mutualistic partnerships with Pinus species. Studied for its systematics, use in sapling nurseries, post-fire seedling establishment, and unique evolutionary history, R. salebrosus holds many secrets within its genome. This thesis aims to characterize the mating system,...
Vietnam is considered one of the most climate change-vulnerable countries due to its high dependence on agriculture and its coastal location. Increased atmospheric temperatures caused by climate change also significantly threaten one of Vietnam's most important cash crops, robusta coffee (Coffea canephora). Suitable cultivation sites of robusta, which comprises 97%...
Bibliographia Bopyridarum is prepared and maintained by John Markham, Arch Cape, Oregon, USA. Any reports of errors or omissions are greatly appreciated. Please contact Dr. Markham with suggestions: jmarkham@seasurf.net This file is updated annually.
Rice (Oryza sativa) is a common crop found in various regions worldwide where it is considered a staple food. Though widespread, O. sativa plants' growth, development, and yield are affected by environmental/abiotic stresses including drought, salinity, flooding, cold, heat, etc. Particularly, salinity stress leads to dehydration, overwhelms potassium pumps, and...
Interactions between proteins are essential to life, driving and regulating a majority of processes within all living cells. Study of protein-protein interactions reveals that some proteins act as hubs within networks of interactions, binding to many partner proteins. These hubs therefore are of particular importance to understanding protein function, interwoven...
Managers of forest and plantation ecosystems are encountering growing problems involving plant pathogens and the expanding geographic ranges of these pathogens. Historically unexposed stands are exceptionally vulnerable when a non-native pathogen is introduced because these pathogens can cause devastating disease in a host population that lacks co-evolved resistance mechanisms. One...
The phosphoprotein (P protein) in viruses of the Mononegavirales order is known to play an essential role in the completion of viral replication mechanisms. The P protein is involved in stabilizing the viral polymerase and nucleoprotein during RNA transcription and replication, regulating nascent RNA encapsidation by the nucleoprotein, and mediation...
An impediment to use of exotic and bioengineered trees in many places is their propensity for spread by pollen and/or seeds. Our laboratory has been using gene editing to induce mutations in floral genes as means to impart stable and reliable genetic containment when this is desirable from social (markets,...
This dissertation consists of four studies of Populus and Eucalyptus biotechnology and genomic science: 1) induction of floral sterility by tapetal expression of the ribonuclease Barnase in Populus; 2) CRISPR Cas9-mediated gene editing targeting LEAFY (LFY) and AGAMOUS (AG) homologs in Populus; 3) induction of floral sterility by CRISPR Cas9...
This dissertation is comprised of four studies on different elements of Populus biotechnology and genomic science: 1) growth improvement by recombinant DNA (rDNA) modification of gibberellin (GA) metabolism; 2) gene editing using zinc-finger nucleases (ZFNs); 3) induction of floral sterility by RNA interference (RNAi) of AGAMOUS (AG) gene homologs; and...