The accuracy of DNA synthesis depends on the accuracy of the polymerase as well as the quality and concentration(s) of the available 5'-deoxynucleoside-triphosphate DNA precursors (dNTPs). The relationships between dNTPs and error rates have been studied in vitro, but only limited insights exist into these correlations during in vivo replication....
Although the Escherichia coli host has almost all of the enzymes necessary to
synthesize nucleotides needed for bacteriophage T4 DNA replication, phage genes
expressed early in infection encode enzymes for de novo DNA precursor biosynthesis
and salvage from degraded host DNA. Eight early enzymes and two host enzymes
comprise the...
The purpose of this study was to isolate and characterize any
segregants of the heterogenotes formed from sexduction by an F'
donor. Following the mating of F' W4520, carrying an F' gal₁⁺
gal₂⁺ factor, with W3350, a gal₁⁻ gal₂⁻ recipient, a number of
recombinant clones were picked and examined for...
With respect to a multienzyme complex of deoxyribonucleoside triphosphate (dNTP) synthesis somehow juxtaposed with DNA replication sites, our laboratory has demonstrated the existence of a multienzyme complex in T4-infected E. coli, named the T4 dNTP synthetase complex, but the idea of direct linkage of dNTP synthesis to DNA replication and...
Protein kinases are an abundant class of enzymes which play important roles in numerous signal transduction systems. Arabidopsis TOUSLED kinase is a serine/threonine kinase which is essential for cell-cell communication within the shoot meristem. TOUSLED is encoded by a single gene in Arabidopsis. Recessive mutants in this gene show mild...
This dissertation describes the cloning and characterization of the TOUSLEDlike kinases genes of maize (ZmTLKs). The TOUSLED-like kinases (TLKs) are a conserved family of nuclear Ser/Thr kinases in higher eukaryotes. The maize genome has three TOUSLED-like kinase genes (ZmTLK1, ZmTLK2, and ZmTLK3). Based upon sequence similarity, the ZmTLKs are divided...
To begin the development of a research tool for use in the in
vivo study of S-adenosylmethionine (SAM) metabolism, I attempted
to locate, isolate, clone and elicit the expression of the SAM
hydrolase gene (E.C. 3.3.1.2) of the coliphage T3.
The gene was isolated and cloned into the M13mp8 cloning...
Ribosomes are intricate macromolecular complexes which are a major
element of the protein biosynthetic machinery in all life forms. In
Escherichia coli they contain about 50 distinct proteins and 3
ribosomal RNAs. The small 30S ribosomal subunit in E. coli
incorporates 21 proteins and a 16S rRNA. The 16S rRNA...