The field performance of genetic containment technologies–considered important for certain uses of transgenic trees in forestry–is poorly known. We tested the efficiency of a barnase gene driven by the TA29 tapetum-dominant promoter for influencing growth rate and inducing male sterility in a field trial of transgenic hybrid poplar (Populus tremula × Populus...
The Middle Fork John Day Basin in Northeastern Oregon is prime habitat for spring Chinook salmon and Steelhead trout. In 2008, a major tributary supporting rearing habitat, Big Boulder Creek, was restored to its historic mid-valley channel along a 1 km stretch of stream 800 m upstream of the mouth....
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Yaker, EstefaniaElorriaga,
Ryan Stewart, Kyle Chambers, Chris Gregory, Daniel Moreno, and many more. I
Water temperature in rivers and streams is an important factor for aquatic ecosystem health. Measurement of stream temperature has traditionally been accomplished by point temperature measurements, continuous point temperature loggers, and more recently, airborne remote sensing techniques such as Forward-Looking Infrared Radar (FLIR) or Thermal Infrared Radiometry. While each of...
This dissertation consists of four studies of Populus and Eucalyptus biotechnology and genomic science: 1) induction of floral sterility by tapetal expression of the ribonuclease Barnase in Populus; 2) CRISPR Cas9-mediated gene editing targeting LEAFY (LFY) and AGAMOUS (AG) homologs in Populus; 3) induction of floral sterility by CRISPR Cas9...
This document describes the design and deployment of a first generation water vapor density sensing unit, the HumiSense. This device is based on an open, air-filled capacitor which is part of a resonant circuit. The frequency of the resonant circuit mixed with a fixed frequency oscillator is the basis of...
Septoria canker remains the most important disease of poplars in intensively managed forest plantations. Genetic resistance has long been considered the best way to manage for this disease. Transgenic resistance mediated by RNA silencing against pathogens and pests (HIGS: host-induced gene silencing) has shown promise in other pathosystems but has...
This dissertation is comprised of four studies on different elements of Populus biotechnology and genomic science: 1) growth improvement by recombinant DNA (rDNA) modification of gibberellin (GA) metabolism; 2) gene editing using zinc-finger nucleases (ZFNs); 3) induction of floral sterility by RNA interference (RNAi) of AGAMOUS (AG) gene homologs; and...
An impediment to use of exotic and bioengineered trees in many places is their propensity for spread by pollen and/or seeds. Our laboratory has been using gene editing to induce mutations in floral genes as means to impart stable and reliable genetic containment when this is desirable from social (markets,...
The field performance of genetic containment technologies–considered important for
certain uses of transgenic trees in forestry–are poorly known. We tested the efficiency of a
barnase gene driven by the TA29 tapetum-dominant promoter for influencing growth rate and
inducing male-sterility in a field trial of transgenic hybrid poplar (Populus tremula x...
The field performance of genetic containment technologies–considered important for
certain uses of transgenic trees in forestry–are poorly known. We tested the efficiency of a
barnase gene driven by the TA29 tapetum-dominant promoter for influencing growth rate and
inducing male-sterility in a field trial of transgenic hybrid poplar (Populus tremula x...