Twenty-six Holstein and 18 Jersey multiparous cows in a randomized block design were assigned by calving date and breed to a non-anionic prepartum diet (CTRL) or a treatment diet containing a commercial anionic salt supplement (Animate®). Control and treatment diets were formulated to a dietary anion-cation difference (DCAD) of 21.3...
Calcium activated proteases, or calpains, are activated in cardiac muscles under conditions of ischemia/reperfusion and hypoxia. Their activity in skeletal muscle under similar conditions is poorly understood. We tested the hypothesis that hypoxia elevates calpain-mediated proteolysis in isolated glycolytic (extensor digitorum longus, EDL) and oxidative (soleus, SOL) mouse muscles studied...
Relationships between myosin heavy chain (MHC) isoform content and
eccentric-induced damage were investigated in chemically skinned muscle fiber
segments. Fibers were subjected to an eccentric contraction standardized for strain
magnitude (0.25 fiber length) and velocity (0.50 maximal shortening velocity).
Fiber MHC isoform content was identified by SDS-PAGE. Pre- to post-eccentric...
This study was designed to determine whether
dietary protein is a nutritional factor influencing the
bioavailability of dietary fluoride. To accomplish
this, a factorial experiment was conducted with
weanling rats fed a purified diet to determine the
influence of dietary protein type (casein or
lactalbumin) and level (12% or 36%)...
The major purpose of this research was to characterize partially degraded proteins
appearing in the ocular lens during aging and cataract, and to identify the responsible
proteolytic activities. This research is significant, because increased protein
degradation is associated with lens opacification and cataract. Determining the sites
where lens proteins become...
Rainbow trout cystatin C cDNA has been isolated from
trout liver. The full-length cystatin cDNA (674 bp)
included the 5'untranslated region and the polyadenylation
signal sequence AATAAA in the 3' region. Translation of
the cDNA defines 132 amino acid residues. Comparison of
the amino acid sequence with those of family...
The aim of this research was to gain more understanding on how a linker histone (LH), H1⁰, and high mobility group protein, HMG1, interact with nucleosome. To determine the location of linker proteins, three specific positioning sequences were used to reconstitute core nucleosomes and the binding of H1 and HMG1...