Calcium activated proteases, or calpains, are activated in cardiac muscles under conditions of ischemia/reperfusion and hypoxia. Their activity in skeletal muscle under similar conditions is poorly understood. We tested the hypothesis that hypoxia elevates calpain-mediated proteolysis in isolated glycolytic (extensor digitorum longus, EDL) and oxidative (soleus, SOL) mouse muscles studied...
The major purpose of this research was to characterize partially degraded proteins
appearing in the ocular lens during aging and cataract, and to identify the responsible
proteolytic activities. This research is significant, because increased protein
degradation is associated with lens opacification and cataract. Determining the sites
where lens proteins become...
In the first experiment the minimum dietary toxic levels of vitamin A were determined in pregnant rabbits over three parities. The treatments consisted of basal diet plus 10,000, 30,000, 60,000, and 90,000 IU/Kg added vitamin A. The basal diet was formulated to be free of vitamin A and beta-carotene; rice...
The aim of this research was to gain more understanding on how a linker histone (LH), H1⁰, and high mobility group protein, HMG1, interact with nucleosome. To determine the location of linker proteins, three specific positioning sequences were used to reconstitute core nucleosomes and the binding of H1 and HMG1...
Little is known about cactus nutrients, their
proportions in different species and the variability of
these proportions with season. In the scant literature
available on Opuntia species, no systematic survey has
been published to document and compare the nutritional
qualities of different species. Also, no studies have
been done to...
Rainbow trout cystatin C cDNA has been isolated from
trout liver. The full-length cystatin cDNA (674 bp)
included the 5'untranslated region and the polyadenylation
signal sequence AATAAA in the 3' region. Translation of
the cDNA defines 132 amino acid residues. Comparison of
the amino acid sequence with those of family...
Relationships between myosin heavy chain (MHC) isoform content and
eccentric-induced damage were investigated in chemically skinned muscle fiber
segments. Fibers were subjected to an eccentric contraction standardized for strain
magnitude (0.25 fiber length) and velocity (0.50 maximal shortening velocity).
Fiber MHC isoform content was identified by SDS-PAGE. Pre- to post-eccentric...