Fungal hyphae are among the most highly polarized cells. Hyphal polarized growth is supported by tip-directed transport of secretory vesicles, which accumulate temporarily in a stratified manner in an apical vesicle cluster, the Spitzenkörper. The exocyst complex is required for tethering of secretory vesicles to the apical plasma membrane. We...
For the past several decades, amphibian populations have been decreasing
around the globe at an unprecedented rate. Batrachochytrium dendrobatidis (Bd), the fungal
pathogen that causes chytridiomycosis in amphibians, is contributing to amphibian declines.
Natural and anthropogenic environmental factors are hypothesized to contribute to these
declines by reducing the immunocompetence of...
The use of high throughput molecular methods that allow for the study of bacterial communities in environmental samples is commonplace in microbial ecology. Until recently, fungal community ecology has
focused on isolation, collection of sporocarps, or collection of ectomycorrhizal roots. The techniques used to extract and amplify DNA from environmental...
Contributing to the worldwide biodiversity crisis are emerging infectious diseases, which can lead to extirpations and extinctions of hosts. For example, the infectious fungal pathogen Batrachochytrium dendrobatidis (Bd) is associated with worldwide amphibian population declines and extinctions. Sensitivity to Bd varies with species, season, and life stage. However, there is...
Coastal Douglas-fir (Pseudotsuga menziesii var. menziesii), a keystone species in the Pacific Northwest, relies on symbioses with microorganisms to obtain nutrients, potentially defend against pathogens, and perform a variety of other tasks. Two of the tree’s primary fungal symbionts are foliar epiphytes and endophytes, which reside on the exterior or...
The isoprenoid pathway is one of the major biosynthetic pathways of secondary metabolites. Isoprenoids with a C₂₀ skeleton are known as diterpenoids and are derived from the common precursor, geranylgeranyl diphosphate (GGPP). This dissertation describes approaches to discover the diterpene synthase enzymes which provide the backbone structures for pleuromutilin and...
This study was initiated to determine the tolerance to copper
of Aspergillus niger, Stemphylium sarcinaeforme, Monilinia
fructicola and Fusarium oxysporum f. sp. lycopersici. Included
were strains of the latter two fungi that were induced to tolerate
relatively high levels of copper by continual culture on sublethal
levels of the toxicant....
The fungi that cause mold and stain of freshly sawn lumber result in millions of dollars in losses to the forest products industry. Stain and mold are typically prevented by either kiln drying or by application of a topical fungicide. While these treatments are effective, kiln drying is costly and...