The role of synthesis and degradation in controlling enzyme
concentrations was studied.
Concentrations of chloride and arginine were determined for
blood and liver at various stages of development from the embryo to
the 8 day old chicken. The chloride concentrations were used to
obtain the volume of extracellular blood in...
Preliminary toxicity studies of BCNU in rats revealed that significant
prolongations of pentobarbital hypnosis were associated with
all doses employed. Investigations were undertaken to determine the
underlying mechanism responsible for this observed effect. Studies
on the in vitro metabolism of pentobarbital by hepatic microsomes
of BCNU-treated animals demonstrated that significant...
Neuromuscular physiology has been extensively investigated
in several groups of vertebrates excluding fish. To
understand the nature of neuromuscular transmission in this
group, the response of the pectoral fin abductor muscle of
black bass (Micropterus salmoides) to nerve stimulation
under the influence of an anticho1inesterase agent was
studied. Diisopropylfluorophosphate (DFP),...
The mechanism(s) involved with intrauterine migration of the porcine embryo was investigated. Twenty-four gilts on Days 6, 9 and 12 of gestation (Day 0 = 1st day of estrus) were utilized to examine the relationship between myometrial activity and intrauterine migration. On Day 2, embryos were flushed from one oviduct...
NADPH-cytochrome P-450 reductase, an important enzyme in the
cytochrome P-450-dependent microsomal monooxygenase system, was
purified from insecticide-resistant (R) and -susceptible (S) house
flies (Musca domestica, L.) and from the black blow fly (Phormia
regina, Weigen). Proteolytic degradation to a P-450 - inactive form
of the reductase was reduced during the...
A sucrose density-gradient ultracentrifugation technique for
obtaining microsomes has been developed. This technique has
advantages over the classic differential centrifugation technique, in
that it is faster, is better suited to work with small quantities of
tissue, and offers the opportunity to work with a defined microsomal
sub-cellular fraction, rather than...