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An impediment to use of exotic and bioengineered trees in many places is their propensity for spread by pollen and/or seeds. Our laboratory has been using gene editing to induce mutations in floral genes as means to impart stable and reliable genetic containment when this is desirable from social (markets,...
This dissertation consists of four studies of Populus and Eucalyptus biotechnology and genomic science: 1) induction of floral sterility by tapetal expression of the ribonuclease Barnase in Populus; 2) CRISPR Cas9-mediated gene editing targeting LEAFY (LFY) and AGAMOUS (AG) homologs in Populus; 3) induction of floral sterility by CRISPR Cas9...
This dissertation is comprised of four studies on different elements of Populus biotechnology and genomic science: 1) growth improvement by recombinant DNA (rDNA) modification of gibberellin (GA) metabolism; 2) gene editing using zinc-finger nucleases (ZFNs); 3) induction of floral sterility by RNA interference (RNAi) of AGAMOUS (AG) gene homologs; and...
Natural durability remains one of the most attractive characteristics of wood, and helps wood obtain a premium price. A worldwide shift towards the use of younger trees from intensively managed forests has created greater concerns about wood quality, especially the wood’s resistance to fungi and insects. Wood durability is assessed...
The field performance of genetic containment technologies–considered important for
certain uses of transgenic trees in forestry–are poorly known. We tested the efficiency of a
barnase gene driven by the TA29 tapetum-dominant promoter for influencing growth rate and
inducing male-sterility in a field trial of transgenic hybrid poplar (Populus tremula x...
The field performance of genetic containment technologies–considered important for
certain uses of transgenic trees in forestry–are poorly known. We tested the efficiency of a
barnase gene driven by the TA29 tapetum-dominant promoter for influencing growth rate and
inducing male-sterility in a field trial of transgenic hybrid poplar (Populus tremula x...
The field performance of genetic containment technologies–considered important for
certain uses of transgenic trees in forestry–are poorly known. We tested the efficiency of a
barnase gene driven by the TA29 tapetum-dominant promoter for influencing growth rate and
inducing male-sterility in a field trial of transgenic hybrid poplar (Populus tremula x...
The field performance of genetic containment technologies–considered important for
certain uses of transgenic trees in forestry–are poorly known. We tested the efficiency of a
barnase gene driven by the TA29 tapetum-dominant promoter for influencing growth rate and
inducing male-sterility in a field trial of transgenic hybrid poplar (Populus tremula x...
Full Text:
male sterility and slows
field growth in Populus
Elorriaga, E., Meilan, R., Ma, C., Skinner, J. S
The stability and value of transgenic pest resistance for promoting tree growth are poorly understood. These data are
essential for determining if such trees could be beneficial to commercial growers in the face of substantial regulatory and
marketing costs. We investigated growth and insect resistance in hybrid poplar expressing the...