Polymerase chain reaction (PCR) primers are reported for 68 nuclear-encoded microsatellites developed during the past several years from genomic libraries of red drum (Sciaenops ocelwtus). All68 microsatellites were tested for reproducibility and polymorphism on a sample of five to 12 red drum; 60 of the microsatellites were found to be...
DNA mismatch repair (MMR) is an evolutionary conserved process that functions to maintain genomic integrity through the correction of mismatches that have escaped proofreading. Mutations in the MMR gene Mlh1 are associated with approximately 50% of all cases of Lynch syndrome, a hereditary predisposition to colorectal cancer, through varying and...
The highly conserved multi-protein mismatch repair (MMR) system is known for its ability to correct post replication errors in genomic DNA. A hallmark of MMR deficiency in all organisms is microsatellite instability. The initiating proteins in the eukaryotic MMR system are hetrodimers formed with an integral MSH2 subunit and one...
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Analysis of Microsatellite Instability.............................................22
Plethodontid salamanders are model organisms for studying evolutionary questions of gene flow, restricted ranges, and speciation. Plethodon, a diverse genus within Plethodontidae, is widely distributed in eastern and western North America. Much research has been based in the eastern United States where diversity of these salamanders is high. Because of...
European Hazelnut (Corylus avellana L.) is an important crop in the Pacific Northwest where around 5% of the world's hazelnuts are produced. Hazelnut production in the Pacific Northwest is threatened by eastern filbert blight (EFB) caused by the pyrenomycete Anisogramma anomala. Host resistance is a useful strategy to control disease,...
We isolated seven polymorphic microsatellite loci from a ground beetle (Bembidion atrocaeruleum, Coleoptera, Carabidae
(Stephens, 1826)) associated with naturally and regularly disturbed floodplain habitat in northwest Europe. Loci were tested on 157 individuals
collected from five distinct habitat patches across two adjacent drainage basins in Wales, United Kingdom, to assess...
Pre-existing Expressed Sequence Tag (EST) libraries (1,305 sequences) and a newly constructed microsatellite-enriched genomic library (136 clones) from the V. corymbosum cultivar 'Bluecrop' were screened for unique Simple Sequence Repeat (SSR)-containing sequences. Our goal was to develop a set of robust SSR markers for use in managing the National Clonal...
The US Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository in Corvallis, Oregon, preserves more than 800 accessions of hazelnut (Corylus), including C. avellana cultivars and representatives of 10 other recognized shrub and tree species. Characterization and study of genetic diversity in this collection require cross-transferable markers, such...
Efforts to restore depressed populations of Pacific salmon Oncorhynchus spp. are often hampered by the inability to assign population identity to individuals in an admixture. This knowledge is of particular concern in supportive breeding programs, in which misidentification of individuals to population may result in progeny of mixed heritage, which,...
European hazelnut, an important nut crop in Oregon agriculture, is threatened by the fungal disease eastern filbert blight (EFB) caused by Anisogramma anomala (Peck) E. Müller. The hazelnut breeding program at OSU has been working on development of EFB-resistant cultivars. DNA markers allow mapping of traits using segregating progenies and...