Relationships between myosin heavy chain (MHC) isoform content and
eccentric-induced damage were investigated in chemically skinned muscle fiber
segments. Fibers were subjected to an eccentric contraction standardized for strain
magnitude (0.25 fiber length) and velocity (0.50 maximal shortening velocity).
Fiber MHC isoform content was identified by SDS-PAGE. Pre- to post-eccentric...
Proteolytic degradation of fish flesh occurring at elevated temperatures is the
primary limitation for the commercial utilization of arrowtooth flounder (ATF).
Characterization of the autolytic activity of ATF muscle incubated at various pHs
and temperatures indicated the involvement of heat-activated proteinases active at
acidic and alkaline pHs. Further characterization of...
Increased sales of surimi seafood, with the majority as crabstick in the United States indicates that surimi based products are becoming more popular. With growing popularity, there is increased competition for market share. Under these circumstances, some companies may be willing to sacrifice product quality in order to facilitate manufacture...
Myoglobin (Mb) was extracted from Pacific sardine and added to Pacific whiting surimi to measure its effects on protein gelation. The purity of Mb extract was determined by SDS-PAGE. Mb extracted using ethanol showed higher purity than Mb extract using ammonium sulfate. The addition of 0.2% Mb significantly reduced breaking...
EF-hand proteins are a conserved family of proteins that are modulated by divalent cations and regulate diverse cellular activities. In the present study we characterized the molecular determinants of myosin regulatory light chain (RLC) interaction with several subunits of the N-methyl-D-aspartate (NMDA)-type glutamate receptor. Myosin RLC is an EF-hand protein...
Prior to cell cleavage, cytokinetic proteins are recruited into the nascent actomyosin contractile ring. Interactions between spindle microtubules and the cell cortex play a critical role in this recruitment. However, direct evidence for physical interaction between microtubules and the cortex has been lacking. Here we reveal the physical connection between...
Myosins are actin-based molecular motors that may have specialized trafficking and contractile functions in cytoskeletal compartments that lack microtubules. The postsynaptic excitatory synapse is one such specialization, yet little is known about the spatial organization of myosin motor proteins in the mature brain. We used a proteomics approach to determine...
Increased competition in the market place for both occasional and professional chain saw users has led to a desire for improved performance in chain saw guide bars. One aspect of improved performance is lower bar weight. The primary focus of this research was to establish the feasibility of a lower...