Nalidixic acid and its derivatives act through inhibition of
bacterial DNA gyrase activity. Recently there have been a series of
papers reporting the antibacterial activity against three different
types of bacteria (S. aureus, E. coli and P. aeruginosa) by a series
of 1,4-dihydro-4-oxoquinoline (or 1,8-naphthyridine) 3-carboxylic
acids. Consistent biological data...
This thesis involves analyses of 3₂-helices, a-helices and of the change in residual
entropy of a protein upon chemical modification. Part of the long-term goal of
understanding the formation, function and stability of proteins requires that all
conformations be accurately assigned and structurally understood. By a statistical
analysis of 258...
A novel protocol for the study of protein-nucleic acid interactions is presented and demonstrated
to be feasible. The protocol combines photochemical crosslinking techniques and mass
spectrometric methods into a new strategy for identifying protein domains or amino acid residues
that are in close contact with nucleic acid in protein-nucleic acid...
The effects of estrogen treatment on uterine weight, uterine
nucleic acid content and adrenal weight were studied. Daily doses
of 0.05μg or 0.1 μg of Estradiol 17-β were administered subcutaneously
to seven day castrated female rats for periods varying
from 0 to 168 days. After two days of treatment uterine...
Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind and silence gene targets. Through designing PPMOs that silence essential or resistance genes in pathogens, these antisense oligomers could be utilized as novel antimicrobials. Towards this end, my thesis employed minimum inhibitory concentration assays (MICs) to identify PPMOs capable of...
Fluorescence of adenine, guanine, thymine, cytosine, and uracil
has been detected at room temperature in neutral aqueous solution
using a digital signal accumulation technique and right angle fluorescence
detection from solutions with absorbances between 0. 4 and 0. 7.
The quantum yields are respectively 2.6 x 10⁻⁴, 3.0 x 10⁻⁴,...
The binding of eleven polycyclic, aromatic hydrocarbons to the acid
form of poly A has been investigated. The hydrocarbons were chosen to
test a size criterion. Model building showed that some hydrocarbons
could intercalate in the helix and be well protected from contact with
the aqueous solvent. Experimentally it was...