The effects of pH on the torsional flexibility of DNA bound to a
nucleosome core particle were investigated by studying the time-resolved
fluorescence anisotropy decays of ethidium bromide intercalated into the DNA
of the core particle. As the torsional flexibility of DNA is affected by the
presence of an intercalating...
The effects of oligoribonucleotides of identical or similar sequences to a
ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly
were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to
the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
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List of Figures
Figure
2.1 The primary and secondary structure of the 16S RNA in E. coli
Nucleic acid estimates in tissues of marine macrophytes with the fluorochromes Hoechst 33258 and ethidium homodimer were severely impaired by high concentrations of interfering compounds. The isolation and extraction techniques used pointed towards compounds of low molecular weight and of polar characteristics that were insensitive to organic solvent extraction and...