For ras gene mutation analysis in the rainbow trout (Oncorhynchus
mykiss) model system, a partial trout ras sequence was identified
using the polymerase chain reaction (PCR). Two synthetic
oligonucleotides based on rat K-ras gene sequence were used as
primers for the PCR procedure. A 90 base pair (bp) sequence,
referred...
A biosystematic investigation of Cryptogramma section
Cryptogramma in western North America has been directed towards
resolution of two separate taxonomic questions.
Results of this research support the hypothesis that a
previously undescribed diploid (2n = 30 II) taxon, C. cascadensis, is
present along with the common and widespread C. acrostichoides....
The Baculoviridae is a family of DNA viruses which are obligate
pathogens for a variety of insects. Baculovirus strains came to the
attention of researchers because they provided a nonchemical
alternative for the biological control of insect pests of agriculture
and forestry (Ignoffo, 1968); however, recently they have been studied...
Sequence-specific PCR markers were derived from one new and eight previously identified random amplified polymorphic DNA (RAPD) markers for the purpose of identifying grape (Vitis) rootstocks. The markers were developed because the RAPD assay was found to be inconsistent and the original RAPD markers unreliable. Southern hybridization analysis of the...
A strain of the phytopathogenic bacterium Pseudomonas syringae
pv. phaseolicola, a pathogen of common bean Phaseolus vulgaris,
harbors a 150 kilobase pair (kb) plasmid pMC7105. In one strain,
LR719, pMC7105 is stably integrated in the bacterial chromosome.
Imprecise excision of the pMC7105 replicon has been shown to result
in the...
A pathogenicity locus of Pseudomonas syringae pv.
syringae identified by Tn5 mutagenesis was investigated.
The mutant strain PS9024 is attenuated for disease
expression in its host, Phaseolus vulgaris, but produces
the hypersensitive reaction (HR) in the nonhost, tobacco
(Nicotina tabacum). A cosmid clone carrying 16 kilobases
(kb) of contiguous genomic...