Monoclonal antibodies were developed against serotype 3, P-1059 strain of Pasteurella multocida. Enzyme-linked immunosorbent assays (ELISA) were used to detect antibodies in supernatants of hybridoma cells to either a surface protective (2.5 S) antigen prepared from a 2.5% saline extract or lipopolysaccharide (LPS) prepared by the phenol-water method. Forty two...
Five experiments were conducted to investigate the
effect of dietary fish meal on the incidence of Sudden Death
Syndrome (SDS) in broiler chickens. Growth, feed
efficiency, total mortality and mortality due to SDS were
evaluated. Necropsy examination was performed on broilers
suspected of dying from SDS. Liver lipid and fatty...
The detection and identification of Newcastle disease
virus (NDV) has never been straightforward. Serologically
indistinguishable pathotypes produce a wide spectrum of
disease that ranges from a severe respiratory, neurologic,
or enteric disease with high mortality to an asymptomatic
infection. Extensive use of ND vaccine strains further
complicates the serological differentiation...
Monoclonal antibodies were developed against USDA
challenge strain of infectious laryngotracheitis virus (ILTV).
Indirect immunofluorescence test was used to detect antibodies
in supernatants of hybridomas. Hybridoma cells were developed
by fusing Sp 2/0 myeloma cells with spleen cells obtained from
mice immunized four times with partially purified USDA
challenge strain...
Seven experiments were carried out to investigate the
involvement of carbohydrate and lipid metabolism on the
incidence of Sudden Death Syndrome (SDS) in broiler
chickens.
Hepatic arachidonate level decreased after 1 h postmortem.
The decreased level of hepatic arachidonate
previously observed in SDS birds was, therefore, associated
with the post-mortem...