Rat selenoprotein W (SeW) promoter activity was investigated using different concentrations of cadmium, copper, and zinc. Two fragments (404bp and 1265bp) of the SeW promoter, containing a single metal response element (MRE), were ligated into the multiple cloning site of a pGL3-Basic reporter plasmid. The constructs were transfected into cultured...
Cell culture systems have provided many insights into
eukaryotic gene expression and other biochemical
mechanisms. Since the cell represents the smallest living
unit of any organism it provides a desirable in vitro
system, allowing biochemical studies without the complex
physiology of an entire animal. However, processes
involving intracellular mechanisms, such...