The development of simple, rapid, and sensitive methods for thiophenol detection is of great importance in various fields, including environmental monitoring and food quality. Here, we present a novel approach for the colorimetric detection of thiophenols using gold nanoparticles (AuNPs) as the sensing platform. AuNPs aggregate in the presence of...
The concept of reducing laboratory operations into microfluidic devices has been an attractive area of research for several decades. Due to several advantages of porous-media microfluidic devices including low mass, portability, power-free fluid transport, and the ability to store dried reagents in the porous structures, these devices show great promise...
The detection of biomolecules is critical for clinical diagnostics but requires specialty labor and high cost. The use of microfluidic paper-based analytical devices (µPADs) for bioassays is simpler and cheaper but has low sensitivity. Improved immobilization of antibodies onto cellulose is demonstrated through surface functionalization with APTES and glutaraldehyde. The...
Electrodeposition is a versatile polymer deposition technique to create nano-microscale materials using an electrical field generated from a charged droplet of solution and a grounded collector. Electrospinning or electrospraying can occur during electrodeposition, leading to the creation of nanofibers or bead-like materials depending on the process parameters. Photopolymerization of the...
A simple Janus-type membrane-based wicking microfluidic device fabricated on a polycaprolactone-filled glass microfiber membrane enables separation and collection of blood plasma or serum from whole blood. This device bridges the wicking and cut-capillary-based solution transport mechanisms in a single membrane. The initial portion of the device is fabricated using selective...
High Performance Liquid Chromatography-Mass Spectrometry coupled with filtration, centrifugation, and other common sample preparation techniques offers a rapid and robust method for separating and quantifying propofol, a commonly used anesthetic, from human blood and its synthetic analogs. Method development is important for not only optimizing separation and quantification for a...
Electrospinning and electrospray are both nano-scale material fabrication techniques, based on related phenomena of electrically charged fluid spray from a droplet of liquid. Material is dissolved in liquid, then a spray is generated by applying a high voltage, creating an electric field, between the fluid dispensing capillary and a grounded...
Analytical techniques are utilized in a wide variety of applications. Traditionally, analytical measurements are achieved by trained personnel in a laboratory setting using expensive scientific instruments, which limits their applicability in resource-limited areas and point-of-care applications. Therefore, the concept of enabling these laboratory-based qualitative and quantitative assays in microfluidic device...
The Micralyne microfluidic toolkit (μTK) was an instrument built by Micralyne in the early 2000’s. It was built for electrophoresis and has a sensitive laser-induced fluorescence detection system. The updates to the system were upgrading the control GUI from running in LabVIEW 5 (Windows XP, 2000) to LabVIEW 2012 (Windows...
Analytical chemistry is an area of chemistry primarily focused on the study and use of instruments for separation, identification, and quantification of an analyte of interest. Specifically, separation science within analytical chemistry often refers to the process of dividing mixtures into their small component parts based on differences in their...