Orthopaedic soft tissue degenerative diseases significantly affect the population, especially as it ages. Degeneration is characterized by a progressive loss of extracellular matrix (ECM) components necessary for the proper functioning of the tissues and joints, eventually leading to pain and dysfunction. In humans, the intervertebral disc (IVD) and tendon are...
Despite the many advances in biotechnology and biomedical engineering, the need for long term organ and tissue storage remains among the unsolved problems in the field. Researchers are investigating several possible solutions to the issue that aim to maintain the function and integrity of the biological tissues during storage and...
While exercise can be prescribed for improving cardiovascular and muscle health, no prescription exists for increasing bone mass. Because bone deformation rate has been identified as an important variable related to osteogenesis, estimates of skeletal loading during human activities likely characterize the associated osteogenic stimulus. However, estimates of body segment...
Hip fractures have high mortality and morbidity rates after incidence, with osteoporosis being a major risk factor due to the loss of bone mineral density (BMD). Lower-body resistance exercises, such as squats, can provide sufficient loading on the hip to induce osteogenic effects. However, this loading may depend on how...
The emerging field of regenerative medicine is mainly approached by two different aspects. First is the use of stem cell based models to generate a suite of differentiated cells for therapeutic applications and the alternative approach is to utilize the non-mammalian models that have the inherent capacity to regenerate their...
Plants develop a vast array of cell shapes and sizes by selectively modifying their surrounding cell walls to expand in some regions and not in others. This process of morphogenesis requires the delivery of secretory vesicles to specific locations at the cell periphery, where exocytosis adds new membrane and proteins...
INTRODUCTION: Tissue engineering is a new methodology for addressing meniscal
injury or loss. Synovium may be an ideal source of cells for in vitro meniscal fibrocartilage
formation, however, favorable in vitro culture conditions for synovium must be
established in order to achieve this goal. The objective of this study was...
Cryoprotectants (CPAs) such as glycerol and dimethyl sulfoxide (DMSO) are commonly used during cryopreservation of cell based therapeutics. Although these additives are beneficial during freezing, it is often desirable to remove them before infusion into a patient. Currently, the most common method for CPA removal is by centrifugation. This method...
The agricultural production of peppermint has been shown to contribute significant quantities of nitrate-nitrogen to groundwater recharge. In an effort to provide new tools for increasing nitrogen efficiency within peppermint production, three research questions were proposed: i) How should plant tissue samples be collected to achieve the greatest precision when...