I. Frozen bovine semen samples were measured by flow cytometry
(FCM) to determine if FCM analyses could be useful for sperm quality
evaluation and prediction of bull fertility. Fluorescent dyes
utilized were acridine orange, to measure sperm nuclear chromatin
heterogeneity by quantifying susceptibility of the chromatin to
denaturation, and rhodamine...
The effect of pyridoxine (PN) supplementation on lymphocyte
responsiveness was investigated in 15 elderly volunteers (aged
65-81 years) by measuring lymphocyte proliferation to T and B cell
mitogens, lymphocyte subpopulations with monoclonal antibodies
(T3, T4, T8) and plasma pyridoxal 5'-phosphate (PLP) concentration
at pre-supplementation and after 1 and 2 months...
A rapid nuclear isolation technique was adapted in
order to examine the question of DNA precursor
compartmentation in mammalian cells. By using this method
a reproducible proportion of the cellular nucleotides
remained associated with the isolated nuclei.
Examination, at several different cell densities, of
exponentially growing HeLa cells showed that...
Published May 1987. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Vaccinia virus-infected animal cells have been used to
study the interactions between the replication of
deoxyribonucleic acid (DNA) and the biosynthesis of its
nucleotide precursors. Some antimetabolites that inhibit
DNA replication have as their targets enzymes of nucleotide
biosynthesis. Furthermore, the disruption of nucleotide
metabolism can alter the fidelity of...
In vivo generation of cytotoxic T cell activity
against alloantigen is suppressed by the aromatic
hydrocarbon (Ah) receptor binding 3,3',4,4',5,5'-
hexachlorobiphenyl isomer (HxCB). Previous studies in
this laboratory suggest that HxCB may alter early
event(s) in the activation of cytotoxic T lymphocytes
(CTL). Production of and response to interleukins 1...
Experiments were conducted to examine the effects of a tumor-promoting phorbol ester, a calcium ionophore and indomethacin on bovine luteal oxytocin (OT) synthesis and secretion and progesterone secretion. Six 18-month-old crossbred beef heifers in Exp. 1 and five similar heifers in Exp. 2 were observed twice daily for estrus using...
Although the synthesis of DNA precursors is
closely coordinated with DNA replication, it is still
not clear whether deoxyribonucleoside triphosphates
(dNTPs) influence DNA replication independently of
their interactions with DNA polymerase catalytic sites.
In an effort to understand the extent to which rate and
fidelity of DNA replication are regulated...