Fluorescence polarization measurements on beef liver glutamate
dehydrogenase conjugated with pyrene butyric acid demonstrate that
the association of the enzyme is adequately described by a reversible
indefinite association with a single equilibrium constant. The data
obtained with protein concentrations up to 0.7 mg/ml in 0.05 M
potassium phosphate, pH 7....
The interaction of skeletal muscle phosphofructokinase
with a variety of acidic proteins including calmodulin and
troponin C and with nucleic acids (RNA and DNA) is manifest
in a reversible, time-dependent loss of catalytic activity.
The inactivation is affected by a number of factors.
Substrates (fructose-6-phosphate and ATP), positive
effectors (fructose-2,6-bisphosphate,...
The consistent application of phosphatase inhibitors and a novel final purification step using a connected series of DE-51, DE-52, and DE-53 anion exchange chromatography columns facilitate the preparation of electrophoretically homogeneous sub-pupulations of rabbit muscle phosphofructokinase which differ in their catalytic properties and endogenous covalent phosphate contents. A band of...
This thesis examines the interconnection between the in vitro and in
vivo phosphorylation of rabbit muscle phosphofructokinase. The first goal
of the project was to show whether a novel site of rabbit muscle
phosphofructokinase that is subject to in vitro phosphorylation, serine 376,
may also become phosphorylated in vivo. Evidence...
In terms of production forestry, more often than not any species that is not the crop
species is considered a competitor as they are using finite growing resources that would
otherwise be available to the crop species. With specific regard to Douglas-fir
(Pseudotsuga menziesii (Mirb.) Franco) production in the Pacific...
Protection of the quality and integrity of food supplies is of global concern. Crops can accumulate non-nutritive and sometimes toxic metals and metalloids. Accumulated metals/metalloids can come in part, from fertilizers, which may contain variable levels of non-nutritive metals or metalloids such as arsenic (As), cadmium (Cd), lead (Pb), nickel...
The thermal denaturation of proteins has been extensively studied using several methods including differential scanning calorimetry (DSC). A custom-built optical system was used to study thermal effects on protein as an alternative method to DSC measurements. It was used to investigate the thermal stability of bovine serum albumin (BSA) with...
