The cytolytic properties of the mosquitocidal 25 Kd protein deltaendotoxin of Bacillus thuringiensis subsp. israelensis (Bti) was studied using the human erythrocyte as a target cell. Both a fixedtime and a kinetic hemolytic bioassay were developed in order to screen a variety of treatments for their influence on the toxinerythrocyte...
Interactions between Bacillus sphaericus and Bacillus
thuringiensis israelensis (Bti), persistence of insecticidal
activity in the mosquito habitat, and suitability of enzyme-linked
immunoabsorbent assay (ELISA) for detecting and
quantifying bacterial endotoxin in the mosquito habitat were
investigated. Interactions between the two bacterial
species were examined by laboratory and field bioassays with...
Cooperative Forestry and Pest Management conducted a pilot control project in central Montana to determine the effectiveness of two commercial formulations of BaaiUus th,uringiensis Berliner applied at 8 billiori international units (BIU) per acre (19.8 BIU per hectare) when used under operational conditions against the western spruce budworm, Choristoneu:roa ocaidentalis...
To develop an assay to detect antibodies to Bacillus
thuringiensis subsp. israelensis in mallard ducks, a growth
curve was first established for the bacterium. The growth
curve indicated that the crystal delta endotoxin would be
best harvested from the rest of the cell material after 12
hours of growth. The...
Dipel (R) was applied by helicopter to three blocks of Douglas fir and Engelmann spruce infested with Choristoneura occidentalis Free., in southwestern Montana. Dosage was 1 pound (7.2 Bill) in 2 gallons of water/acre (453 g in 7.5 t/0.4 ha). A commercial surfactant, Bio-film (R) was added at the rate...
The microbial insecticide, Bacillus thuringensis (Bt), was applied from fixed-wing aircraft to approximately 700 acres of western spruce budworm host type at the rate of 12 billion international units (BIU's) per gallon of water per acre. The objective of this control project was foliage protection to insure future cone production...
This evaluation showed that Bt provided measurable foliage protection 1 year after treatment, but no differences were evident after 2 years. We recommend future Bt tests include postspray population measurements in the pupal and/or adult, and/or egg stage, and that foliage protection be measured the following year. The effects of...
1. Defoliation by cankerworms in 1979 was significantly less in 1978 treated shelterbelts than in untreated shelterbelts. 2. Most shelterbelts treated in 1978 retained enough foliage by late June 1979 so that adjacent crops were protected from wind. 3. Aerially applied Bt at a rate of 1/2 lb./acre provided shelterbelt...