Aquifers are an important storage location and source of fresh groundwater. They may become polluted by a number of contaminants including mobile divalent radionuclides such as strontium-90 which is a byproduct of uranium fission. A method for remediating such divalent radionuclides is sequestration through co-precipitation into calcium carbonate. Calcium carbonate...
Saccharomyces cerevisiae strain XS-380, a. heteroallelic
diploid strain, carries six genetically analyzed loci on the left arm of
chromosome VII. Nitrosoguanidine (NMG) at low concentrations was
observed to cause allelic recombination (either mitotic recombination
or gene conversion) of the heteroallelic loci with low lethality.
The NMG-inducible heteroallelic system was used...
The human pathogen Chlamydia trachomatis displays different phenotypes between serovars both in vivo and in vitro. The study of chlamydial biology has been hampered by the lack of a tractable genetic system. Here, chlamydial isolates with known phenotypes as well as strains generated though recombination were fully sequenced to address...
Mycobacterium avium subsp. paratuberculosis (MAP) is a cause of
Johne's disease (JD) in cattle and other ruminants. MAP infection in the bovine
host is not well characterized. It is assumed that crossing the bovine intestinal
mucosa is important for MAP to establish infection. MAP's ability to infect
bovine epithelial cells...
Mycobacterium avium is a ubiquitous environmental organism found in water and soil. It can cause disease in patients with pre-existing pulmonary conditions, immunocompromised patients with the most prevalent being AIDS patients, as well as apparently healthy people. Studies have indicated that, upon macrophage uptake, Al. avium prevents phagosome-lysosome fusion, thus...
The chemolithoautotrophic nitrite oxidizing bacteria (NOB) participate in the
biogeochemical cycling of nitrogen by catalyzing and conserving energy from the
oxidation of nitrite (NO₂-) to nitrate (NO₃-) via a nitrite oxidoreductase (NXR). The
main objective of this work was to comparatively annotate and analyze the genome
sequences of Nitrobacter winogradskyi...
The ability to move towards favorable environmental conditions, called chemotaxis, is common among motile bacteria. In particular aerotaxis has been extensively studied in Escherichia coli. Three putative aer gene homologs were identified in the V. cholerae genome designated VCAer-1 (VC0512) VCAer-2 (VCA0658), and VCAer-3 (VCA0988). Deletion analyses indicated that only...