A new planktonic ecosystem model was constructed for the Eastern Bering Sea based on observations from the 2007–2010 BEST/BSIERP (Bering Ecosystem Study/Bering Sea Integrated Ecosystem Research Program) field program. When run with forcing from a data-assimilative ice-ocean hindcast of 1971–2012, the model performs well against observations of spring bloom time...
Imaging Ca²⁺ dynamics in living systems holds great
potential to advance neuroscience and cellular biology. G-GECO1.1
is an intensiometric fluorescent protein Ca²⁺-biosensor with a Thr-Tyr-Gly chromophore. The protonated chromophore emits green
upon photoexcitation via excited-state proton transfer (ESPT). Upon
Ca²⁺ binding, a significant population of the chromophores becomes
deprotonated. It...
Fluorescent proteins (FPs) are luminescent biomolecules that emit characteristic hues upon irradiation. A group of calmodulin (CaM)-green FP (GFP) chimeras have been previously engineered to enable the optical detection of calcium ions (Ca²⁺). We investigate one of these genetically encoded Ca²⁺ biosensors for optical imaging (GECOs), GEM-GECO1, which fluoresces green...
Fluorescent proteins (FPs) have played a pivotal role in bioimaging and advancing biomedicine. The versatile fluorescence from engineered, genetically encodable FP variants greatly enhances cellular imaging capabilities, which are dictated by excited state structural dynamics of the embedded chromophore inside the protein pocket. Visualization of the molecular choreography of the...
A new continuous wave sonic anemometer was constructed using inexpensive ultrasonic transducers and phase-locked loop integrated circuitry. Vertical wind is measured by sensing the difference in phase between sound waves traveling in the +g and −z directions. The direction of sound travel is reversed 78 times per second, so the...
The kinetics of laser-induced, liquid-mediated crystallization of amorphousGe thin films were studied using multi-frame dynamic transmission electron microscopy (DTEM), a nanosecond-scale photo-emission transmission electron microscopy technique. In these experiments, high temperature gradients are established in thin amorphousGe films with a 12-ns laser pulse with a Gaussian spatial profile. The hottest...
Full Text:
. Santala, S. Raoux, and G.H. Campbell
Figure S1 Zone sizes measured after laser crystallization on
The kinetics of laser-induced, liquid-mediated crystallization of amorphousGe thin films were studied using multi-frame dynamic transmission electron microscopy (DTEM), a nanosecond-scale photo-emission transmission electron microscopy technique. In these experiments, high temperature gradients are established in thin amorphousGe films with a 12-ns laser pulse with a Gaussian spatial profile. The hottest...
Full Text:
, S., & Campbell, G. H. (2015). Kinetics of liquid-mediated
crystallization of amorphous Ge from
The kinetics of laser-induced, liquid-mediated crystallization of amorphousGe thin films were studied using multi-frame dynamic transmission electron microscopy (DTEM), a nanosecond-scale photo-emission transmission electron microscopy technique. In these experiments, high temperature gradients are established in thin amorphousGe films with a 12-ns laser pulse with a Gaussian spatial profile. The hottest...