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  • Processing and functional diversity of plant microRNA

    Creator:
    Cuperus, Josh
    Abstract:
    RNA interference pathways can involve amplification of secondary siRNAs by RNA-dependent RNA polymerases. In plants, RDR6-dependent secondary siRNAs, including transacting siRNAs (tasiRNAs), arise from transcripts targeted by some microRNAs (miRNAs). In the case of TAS3 tasiRNA formation, ARGONAUTE7 (AGO7)-miR390 complexes interact with primary transcripts at two sites, resulting in recruitment...
    Resource Type:
    Dissertation
    Full Text:
    approved: James C. Carrington RNA interference pathways can involve amplification of

  • Rapid evolution of post-transcriptionally regulated RESTORER OF FERTILITY-LIKE genes in the genus Arabidopsis

    Creator:
    Jogdeo, Sanjuro
    Abstract:
    The Pentatricopeptide Repeat (PPR) gene family produces RNA-binding proteins that target organellar transcripts. The PPR family is expanded in land plants, with nearly 450 genes identified in Arabidopsis thaliana. In plants with a Cytoplasmic Male Sterility (CMS) phenotype, members of the PPR family can act as a RESTORER OF FERTILITY...
    Resource Type:
    Masters Thesis
    Full Text:
    FERTILITY-LIKE Genes in the Genus Arabidopsis Abstract approved: James C

  • Origins and evolution of plant microRNA genes

    Creator:
    Fahlgren, Noah
    Abstract:
    Eukaryotic small RNA (~20-30 nucleotides) are diverse regulatory molecules that repress gene expression at the transcriptional and post-transcriptional levels, defend hosts against invading viruses and defend genomes against selfish DNA elements. Small RNA populations are studied by high-throughput sequencing of the total small RNA fraction isolated from cells, however, the...
    Resource Type:
    Dissertation
    Full Text:
    approved: James C. Carrington Eukaryotic small RNA (~20-30 nucleotides) are diverse regulatory

  • Identifying microRNAs by automated phylogenetic and structural analysis

    Creator:
    Smith, Daniel P.
    Abstract:
    Motivation: It can take considerable amounts of time and effort to use existing computational tools to analyze a small RNA sequence for the purpose of determining if it is characteristic of microRNA. It is similarly tedious to search for homologs to known microRNA sequences in the rapidly growing collection of...
    Resource Type:
    Undergraduate Thesis
    Full Text:
    . Abstract approved: Dr. James Carrington Motivation: It can take considerable amounts of time

  • Small RNA pathways in plants

    Creator:
    Montgomery, Taiowa A.
    Abstract:
    miRNA-guided cleavage initiates entry of primary transcripts into the trans-acting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA POLYMERASE6 (RDR6), DICER-LIKE 4 (DCL4), and SUPPRESSOR OF GENE SILENCING3 (SGS3). Arabidopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173- guided initiation cleavage on the 5’ side of the siRNA-generating...
    Resource Type:
    Dissertation
    Full Text:
    . Title: Small RNA Pathways in Plants Abstract approved: James C. Carrington miRNA

  • Endogenous and antiviral RNA silencing pathways in Arabidopsis

    Creator:
    Chapman, Elisabeth J.
    Abstract:
    RNA silencing pathways are required for a wide variety of processes in most eucaryotes. In plants, small-interfering RNA (siRNA) arising from transposons and other repetitive sequences is associated with heterochromatin formation and maintenance. MicroRNAs and trans-acting siRNAs encoded at discrete loci function as negative regulators of gene expression by triggering...
    Resource Type:
    Dissertation
    Full Text:
    James C. Carrington RNA silencing pathways are required for a wide variety of processes in most

  • Chemicals from Douglas-fir bark

    Creator:
    Kurth, E. F. (Ervin Frank) and Oregon Forest Products Laboratory
    Resource Type:
    Technical Report
    Full Text:
    U hemicals crom Douglas-fir Bark by E. F. Kurlh Report No. C

  • Debilitation of Plant Potyvirus Infectivity by P1 Proteinase-Inactivating Mutations and Restoration by Second-Site Modifications

    Creator:
    Verchot, Jeanmarie and Carrington, James C.
    Abstract:
    Tobacco etch virus (TEV) encodes three proteinases that catalyze processing of the genome-encoded polyprotein. The P1 proteinase originates from the N terminus of the polyprotein and catalyzes proteolysis between itself and the helper component proteinase (HC-Pro). Mutations resulting in substitution of a single amino acid, small insertions, or deletions were...
    Resource Type:
    Article
    Full Text:
    VERCHOT AND JAMES C. CARRINGTON* Department of Biology, Texas A&M University, College Station, Texas

  • Evidence that the Potyvirus P1 Proteinase Functions in trans as an Accessory Factor for Genome Amplification

    Creator:
    Verchot, Jeanmarie and Carrington, James C.
    Abstract:
    The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa and HC-Pro proteinases each provide multiple functions essential for viral infectivity, the role of the P1 proteinase beyond its autoproteolytic activity is understood poorly. To determine if P1 is necessary...
    Resource Type:
    Article
    Full Text:
    Functions in trans as an Accessory Factor for Genome Amplification JEANMARIE VERCHOT AND JAMES C

  • The Role of Arabidopsis RNA-Dependent RNA Polymerase Genes 3, 4 and 5 in Antiviral Defense

    Creator:
    Lowery, Nicholas C.
    Abstract:
    RNA silencing plays a critical role in plant defense against viral infection. Plants use virusderived small interfering RNA to target and silence invading viruses. The antiviral silencing pathway can be broken down into three conceptual stages: initiation, amplification, and systemic movement. The molecular mechanisms of silencing initiation are not well...
    Resource Type:
    Honors College Thesis
    Full Text:
    . Abstract Approved: _______________________________________________________ James C. Carrington