The mechanisms of mortality during critical life stages of fish are not well-understood and, for many species, it is not clear if the mechanisms are similar for naturally and artificially propagated individuals. For Chinook salmon (Oncorhynchus tshawytscha), natural fish potentially face negative interactions, such as competition, and survival disadvantages, such...
The purpose of this investigation was to purify and characterize
the autolytic enzyme from cell walls of Bacillus subtilis 168. The
crude enzyme was obtained by autolysis of purified cell walls in
buffer at 37° C. Two purification methods were developed. The first
involved fractional precipitation with ammonium sulfate. The...
The formation of hydrogen peroxide (H₂O₂) by the FAD-dependent α-glycerophosphate oxidase (GlpO), is important for the pathogenesis of Streptococcus pneumoniae and Mycoplasma pneumoniae. The structurally known GlpO from Streptococcus sp. (SspGlpO) is similar to the pneumococcal protein (SpGlpO) and provides a guide for drug design against that target. However, M....
L-α-glycerophosphate oxidase is an FAD-dependent enzyme that catalyzes the oxidation of L-α-glycerophosphate (Glp) by molecular oxygen to generate dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H₂O₂). The catalytic properties of the recombinant His₆-GlpO from Mycoplasma pneumoniae (His₆-MpGlpO) were investigated with transient and steady-state kinetics and ligand binding. The results indicate that...
The underlying causes of mortality during critical life stages of fish are not well
understood, nor is it clear if these causes are similar for naturally versus artificially propagated (i.e.
hatchery) individuals. To assess the importance of selective mortality related to production type
(hatchery vs. naturally produced) and size at...
Large river plumes modify coastal environments and can impact production across multiple trophic levels. From 1999 to 2009, the
assemblages of forage fish, predator fish, and other pelagic nekton were monitored in coastal waters associated with the Columbia
River plume. Surveys were conducted at night to target vertically migrating species,...
Trophic interactions within and among species vary widely across spatial scales and species’ ontogeny. However, the drivers and implications of this variability are not well understood. Juvenile Chinook salmon Oncorhynchus tshawytscha have a wide distribution, ranging from northern California to the eastern Bering Sea in North America, but it is...
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al. 2004). Due to the complexity of model-
ing entire ecosystems, current approaches generally
assume
Oxidized hydrous intermediate composition magmas are responsible for porphyry copper (Cu ±Mo ±Au) deposits and epithermal Au ore deposits formed globally in the shallow crust (Sillitoe, 2010; Seedorff et al., 2005). Recently, zircon geochemistry has been used to characterize both productive and barren intrusions associated with porphyry Cu-Au ore deposits....
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used as a proxy to magmatic crystallization and cooling of the melt (Claiborne
et al., 2006). The
Volcanic and sedimentary deposits of the Mount Jefferson area (MJA) record a fourmillion-year
history of arc-related volcanism related to the subduction of the Juan de Fuca plate
beneath North America. 171 mapped stratigraphic units over an area of 150 km² reveal four
periods of volcanic activity resulting in diverse composition...
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agreement with calculated partition coefficients of
Bindeman et al. (1998) and Dohmen and Blundy (2014