Plants develop a vast array of cell shapes and sizes by selectively modifying their surrounding cell walls to expand in some regions and not in others. This process of morphogenesis requires the delivery of secretory vesicles to specific locations at the cell periphery, where exocytosis adds new membrane and proteins...
BACKGROUND: Exocytosis is integral to root growth: trafficking components of systems that control growth (e.g., PIN
auxin transport proteins) to the plasma membrane, and secreting materials that expand the cell wall to the
apoplast. Spatiotemporal regulation of exocytosis in eukaryotes often involves the exocyst, an octameric complex
that tethers selected...
BACKGROUND: Exocytosis is integral to root growth: trafficking components of systems that control growth (e.g., PIN
auxin transport proteins) to the plasma membrane, and secreting materials that expand the cell wall to the
apoplast. Spatiotemporal regulation of exocytosis in eukaryotes often involves the exocyst, an octameric complex
that tethers selected...
Full Text:
determine
meristem size during primary root growth in
Arabidopsis
Rex A Cole, Samantha A McInally and John E
BACKGROUND: Exocytosis is integral to root growth: trafficking components of systems that control growth (e.g., PIN
auxin transport proteins) to the plasma membrane, and secreting materials that expand the cell wall to the
apoplast. Spatiotemporal regulation of exocytosis in eukaryotes often involves the exocyst, an octameric complex
that tethers selected...
Repetitive sequences present a challenge for genome sequence assembly, and highly similar segmental duplications may
disappear from assembled genome sequences. Having found a surprising lack of observable phenotypic deviations and
non-Mendelian segregation in Arabidopsis thaliana mutants in SEC10, a gene encoding a core subunit of the exocyst
tethering complex, we...
Repetitive sequences present a challenge for genome sequence assembly, and highly similar segmental duplications may
disappear from assembled genome sequences. Having found a surprising lack of observable phenotypic deviations and
non-Mendelian segregation in Arabidopsis thaliana mutants in SEC10, a gene encoding a core subunit of the exocyst
tethering complex, we...
Full Text:
Vukašinović, N., Cvrčková, F., Eliáš, M., Cole, R., Fowler, J. E., et al. (2014)
Dissecting a Hidden Gene
Using genetic approaches, particle image velocimetry and an inert tracer of cytoplasmic streaming, we have made a mechanistic connection between the motor proteins (myosins XI), cargo transported by these motors (distinct endomembrane compartment defined by membrane-anchored MyoB receptors) and the process of cytoplasmic streaming in plant cells. It is shown...
Repetitive sequences present a challenge for genome sequence assembly, and highly similar segmental duplications may disappear from assembled genome sequences. Having found a surprising lack of observable phenotypic deviations and non-Mendelian segregation in Arabidopsis thaliana mutants in SEC10, a gene encoding a core subunit of the exocyst tethering complex, we...
BACKGROUND:
Plant gametophytes play central roles in sexual reproduction. A hallmark of the plant life
cycle is that gene expression is required in the haploid gametophytes. Consequently, many
mutant phenotypes are expressed in this phase.
RESULTS:
We perform a quantitative RNA-seq analysis of embryo sacs, comparator ovules with the
embryo...
BACKGROUND:
Plant gametophytes play central roles in sexual reproduction. A hallmark of the plant life
cycle is that gene expression is required in the haploid gametophytes. Consequently, many
mutant phenotypes are expressed in this phase.
RESULTS:
We perform a quantitative RNA-seq analysis of embryo sacs, comparator ovules with the
embryo...
Full Text:
., Cole, R. A., Coker, C. T., Unger-Wallace, E.,
Vejlupkova, Z., ... & Evans, M. (2014). Discovery of
