Experiments were conducted to determine whether endogenous progesterone regulates synthesis and/or secretion of luteal oxytocin (OT). In experiment 1, mature ewes (n=5 per group) were assigned randomly to control or mifepristone (RU 486) treatment groups. Ewes were injected twice daily s.c. with vehicle or 10 mg RU 486 from days...
Prostaglandin F₂α (PGF₂α), a stimulatory hormone of luteal oxytocin
secretion, is known to activate protein kinase C (PKC); however, the
intracellular signals that promote exocytosis of oxytocin remain to be
elucidated. Myristoylated alanine-rich C kinase substrate (MARCKS), a
protein specifically phosphorylated by PKC, crosslinks actin filaments
associated with the inner...
Rat luteal cells from functional and non-functional
corpora lutea (corpora lutea rendered non-functional by
hypophysectomy) were preserved for light and electron
microscopy in Bouin's and glutaraldehyde-osmium fixation
respectively. Material for electron microscopy was dehydrated
with ethanol, embedded in Araldite and stained
with lead hydroxide or uranyl acetate-lead citrate. Examination
of...
Some effects of 171β-estradiol on ovine luteal function were
studied in five experiments.
Estradiol injected into ewes on days 11 and 12 of the estrous
cycle caused luteal regression in both intact and unilaterally hysterectomized
ewes indicating that the presence of one uterine horn permits
estradiol to cause luteolysis comparable...
Two experiments were conducted to evaluate the effects of
gonadotropin releasing hormone (GnRH) on the function of the bovine
corpus luteum during the estrous cycle. In Experiment 1, 10 heifers
were assigned randomly into two groups with each heifer serving as
her own control. Heifers in Group I (n=5) were...
Two experiments were conducted to investigate the effects of
gonadotropin-releasing hormone (GnRH), administered twice during
the same estrous cycle, on bovine luteal function. Eight mature
beef cows, each cow serving as her own control, were injected
intravenously (i.v.) with saline on days 2 and 8 of the cycle (day of...
Four experiments were conducted to determine oxytocin concen tration of bovine luteal tissue at different stages of the estrous cycle and to study the effects of prostaglandins F₂α (PGF₂α), E₂ (PGE₂), luteinizing hormone (LH), cycloheximide, colchicine and cytochalasin B on oxytocin synthesis and release in vitro. In experiment 1, luteal...
Two experiments were conducted to examine the effect of
a cyclopropenoid fatty acid on luteal cell function. In Exp.
1, 12 mature ewes were mated to a fertile ram, assigned to two
groups (n = 6/group) and laparotomized on day 18 of gestation.
Ewes with corpora lutea (CL) in both...
Experiments were conducted to examine the effects of a tumor-promoting phorbol ester, a calcium ionophore and indomethacin on bovine luteal oxytocin (OT) synthesis and secretion and progesterone secretion. Six 18-month-old crossbred beef heifers in Exp. 1 and five similar heifers in Exp. 2 were observed twice daily for estrus using...