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Creator:: Li, Fugen
Abstract:: A partial cystatin cDNA from rainbow trout was generated by reverse transcription polymerase chain reaction with two degenerate primers. The partial cystatin PCR product was 168 bp and used to screen trout liver λgt 11 cDNA library. Four positive clones were isolated and designated as cstl, cst2, cst3 and cst4....
Resource Type:: Masters Thesis
Full Text:: difference may reflect the regulation of cysteine proteinase activities. Southern blot of trout genomic DNA

Creator:: Akpinar, Ozlem
Abstract:: High proteolytic activity Pacific whiting muscle causes hydrolysis of myofibrillar protein and lowers surimi gel quality. Although food grade proteinase inhibitors can be used to prevent autolytic activity in surimi, their usage is limited due to their adverse effects on the organoleptic quality of surimi. Cystatins however can be used...
Resource Type:: Masters Thesis
Full Text:: organoleptic quality of surimi. Cystatins however can be used as a specific cysteine proteinase inhibitor

Creator:: Li, Fugen
Abstract:: Rainbow trout cystatin C cDNA has been isolated from trout liver. The full-length cystatin cDNA (674 bp) included the 5'untranslated region and the polyadenylation signal sequence AATAAA in the 3' region. Translation of the cDNA defines 132 amino acid residues. Comparison of the amino acid sequence with those of family...
Resource Type:: Dissertation
Full Text:: Chapter 1. Cysteine Proteinase Inhibitor Cystatin C 1 Cystatin Superfamily 1 Cystatin C Gene

Creator:: Solem, Michele Lee
Resource Type:: Dissertation
Full Text:: ). There are three classes of cysteine proteinase inhibitors. Class I and II are low molecular weight

Creator:: Nickel, Xianbin F.
Abstract:: Proteinase P-II purified from parasitized Pacific whiting muscle was previously identified to be one form of cathepsin L. It appeared to be present in three isozymatic forms on non-denaturing PAGE gel stained for activity. Its autolytic degradation was observed on SDS-PAGE gel under its optimum condition, 55°C and pH 5.5,...
Resource Type:: Masters Thesis
Full Text:: revealed that this cysteine proteinase from Pacific whiting might be encoded by a cathepsin L-related

ScholarsArchive@OSU